摘要
目的建立雪胆胃肠丸中延胡索乙素含量测定的方法。方法采用Hypersil ODS2柱（250 mm×4.6 mm,5μm）,乙腈-0.1%磷酸溶液（三乙胺调pH至6.0）（40∶60）为流动相;流速1.0 mL.min^-1;检测波长225 nm;柱温：25℃。结果延胡索乙素在4.8-48.0μg.mL^-1内与其峰面积有良好的线性关系,其平均回收率为99.82%,RSD为1.00%（n=6）。结论所建方法简便易行、准确可靠,可用于雪胆胃肠丸中延胡索乙素的含量测定。
OBJECTIVE To establish an HPLC method for determination of contents of trahydorpalmatine in Xuedan Weichang pill.METHODS To utilize RP-HPLC,the Hypersil ODS2 column（250 mm×4.6 mm,5 μm） was used with the mobile phase of acetonitrile-0.1% phosphate acid（adjustd to pH 6.0 by triethylamine）（60∶40）.The flow rate was 1.0 mL.min^-1.The detection wavelength was 225 nm.The column temperature was 25 ℃.RESULTS The linear range of trahydorpalmatine was 4.8-48.0 μg.mL^-1.The average recoveries was 99.82% with RSD of 1.00%（n=6）.CONCLUSION The method established is sensitive,accurate and can be used for quality control of Xuedan Weichang pill.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2010年第5期436-439,共4页
Chinese Journal of Modern Applied Pharmacy
基金
国家"十一五"科技支撑计划重点项目子课题(2006BA108B03-05)
