摘要
目的观察两种饮食限制方式下各组大鼠肝脏细胞自噬的程度,以期探索饮食限制与细胞自噬的关系。方法 24月老龄大鼠按体重随机分为A组(正常饮食对照组)、B组(1d自由饮食1d禁食)、C组(5d自由饮食2d禁食),每组10只。饲养5个月后活体状态下取1mm3肝脏组织4℃保存,50mg肝组织-70℃冻存。透射电镜观察各组肝脏细胞自噬泡的数量;RT-PCR检测微管相关蛋1-轻链3(MAP1-LC3)的mRNA表达。结果与A组相比,B组和C组自噬泡占细胞质总面积的比值均明显提高(P<0.01),而两组间该比值差异无统计学意义(P>0.05)。B组和C组MAP1-LC3的表达较A组显著升高(P<0.01),而两组间差异无统计学意义(P>0.05)。结论两种饮食限制方式使大鼠的肝脏细胞自噬程度明显增强,通过饮食限制可增强大鼠肝脏细胞的自噬程度。
Objective To explore the relationship between autophagy of rat hepatocytes and dietary restriction. Methods Thirty 24-month-old rats were randomly devided into three groups：group A （normal diet）,group B （1 d free diet and 1d fasting）,group C （5 d free diet and 2 d fasting）.After feeding for five months,in vivo 1 mm3 liver tissues were taken and stored at 4 ℃,while 50 mg liver tissues were stored at -70 ℃.The amount of autophagosomes of hepatic cells was detected by transmission electron microscope（TEM）.RT-PCR was used to detect the expression of microtubule-associated protein 1-light chain 3（MAP1-LC3）mRNA. Results Compared with group A,the ratio of autophagosomes to that of total cytoplasm increased significantly in both group B and group C（P〈0.01）,but there was no significant difference between group B and group C（P〈0.05）.MAP1-LC3 expression was significantly higher in both group B and group C than in group A（P〈0.01）,while no statistical significance between group B and group C（P〈0.05）. Conclusion The degree of autophagy of hepatocytes obviously increases in rats with dietary restrictions.The results suggest that dietary restrictions can improve the autophagy of rat hepatocytes.
出处
《山西医科大学学报》
CAS
2010年第5期396-399,共4页
Journal of Shanxi Medical University
基金
山西省基础研究计划项目(自然)(2010011054-2)
关键词
细胞自噬
饮食限制
透射电镜
微管相关蛋1-轻链3
autophagy
dietary restriction
transmission electron microscope
microtubule-associated protein 1-light chain 3
