摘要
目的对非霍奇金淋巴瘤的微小病灶（ＭＲＤ）及时发现。方法应用聚合酶链反应－聚丙烯酰胺凝胶电泳（ＰＣＲ－ＰＡＧＥ）和银染色法对２１１例非霍奇金淋巴瘤（ＮＨＬ）进行免疫球蛋白重链基因（ＩｇＨ），Ｔ细胞受体γ链基因（ＴＣＲγ）的克隆性重排检测。结果重排之ＩｇＨ为１０８例（５１．２％），ＴＣＲγ４５例（２１．３％），双阳性１２例（５．７％），阴性４６例（２１．８％）。病理型别恶性程度愈高，ＴＣＲγ及双阳性愈高。原发于鼻及咽淋巴环的ＮＨＬ比胃肠及周围淋巴结的ＴＣＲγ及双阳性高。ＴＣＲγ及双阳性的增高与病情恶化程度呈正相关。结论；
Objective To detect the minimal residuel diseases(MRD) of Non Hodgkin's lymphoma(NLL).Methods The rearrangment of immnuglobin heavy chain gene (TGH)and T cell receptor r chain gene(TCRγ) in all cases of NL were detected by using polymerase chain reaction(PCR),polyacrylamine gel electrophoresis(PAGE)and silver stain.Results The rearrangement of IgH were 108 cases(51.2%),TCRγ 45 cases(21.3%), both positive 12 cases(5.7%) and negative 46 cases(21.8%).The more malignant in pathological type,the more TCRγ and both positive will be.Similarly,the primary sites in nasal or waldeyer's ring were more severe than that in lymph node or gastrointesting.Conclusions The TCRγ and both positive have positive relationship with the severety of diseases.So as to indicate the chemotherapy with this method,it would make a great increase of survival and cure rate.
出处
《肿瘤研究与临床》
CAS
1999年第1期19-21,共3页
Cancer Research and Clinic
关键词
非霍奇金淋巴瘤
微小病灶
基因重排
Non Hodgkin's lymphoma Clonic gene rearrangement Minimal residual diseases
