摘要
目的转化生长因子－β１（ＴＧＦβ１）在细胞生长的负性调节上有重要作用，是肝细胞生长和增殖的强抑制物但肝癌（ＨＣＣ）患者肝组织中ＴＧＦβ１ｍＲＮＡ表达高．现检测ＨＣＣ患者外周血中ＴＧＦβ１ｍＲＮＡ表达，血清ＴＧＦβ１水平，以及肝组织中ＴＧＦβⅡ型受体（ＴＧＦβＲⅡ）的表达．方法ＨＣＣ患者外周血分离单个核细胞（ＰＢＭＣ），提取总ＲＮＡ，用反转录聚合酶链反应（ＲＴＰＣＲ）技术扩增ＴＧＦβ１ｍＲＮＡ．血清ＴＧＦβ１水平测定用Ｐｒｏｍｅｇａ公司产的试剂盒．肝组织ＴＧＦβＲⅡ表达的分析用原位杂交法．结果ＨＣＣ患者２０例ＰＢＭＣＴＧＦβ１ｍＲＮＡ用ＲＴＰＣＲ检测阳性率达７０％，对照组阴性．ＨＣＣ患者４０例血清ＴＧＦβ１水平（２７５８ｍｇ／Ｌ±８１０ｍｇ／Ｌ）明显高于对照组（８２７ｍｇ／Ｌ±３７２ｍｇ／Ｌ）．原位杂交表明，ＴＧＦβＲⅡ在ＨＣＣ细胞的胞质中有弱表达．结论ＨＣＣ患者ＴＧＦβ１基因在转录水平和翻译水平上均显示表达增强．ＰＢＭＣ有可能代替肝组织用以检测ＴＧＦβ１ｍＲＮＡ的表达应用于基础与临床的研究．
AIM Transforming growth factor β1 (TGF β1) is now recognized as playing an important role in negative cell growth regulation. It is a potent inhibitor of hepatocyte growth and proliferation. But high expression of TGF β1 mRNA in the liver tissues from patients with hepatocellular carcinoma (HCC) has been reported recently. The aim of the present study is to detect the expression of TGF β1 mRNA and the serum level of TGF β1 in the peripheral blood as well as expression of TGF β type Ⅱ receptors (TGF β RⅡ) in the tumor cells of patients with HCC. METHODS From 20 patients with HCC and 5 healthy volunteers, peripheral blood mononuclear cells (PBMC) were seperated, total RNA was extracted and TGF β1 mRNA was amplified from it by using RT PCR. The serum level of TGF β1 was determined by using Promega's kit. The expression of TGF β RⅡ was detected by using in situ hybridization. RESULTS The positive rate of PBMC TGF β1 mRNA in HCC patients was 70%, but negative result was found in the healthy volunteers. The serum level of TGF β1 in HCC patients ( 27 58mg/L ± 8 10mg/L ) was significantly higher than in normal control ( 8 27mg/L ± 3 72mg/L , P <0 01). TGF β RⅡ weak expression was observed in the cytoplasm of tumor cells. CONCLUSION TGF β1 gene expression is increased in HCC patients both in transcription level and translation level. PBMC may be possibly used instead of tissue section for TGF β1 mRNA detection in basic and clinical studies.
出处
《世界华人消化杂志》
CAS
1999年第2期150-152,共3页
World Chinese Journal of Digestology
关键词
肝肿瘤
转化生长因子
基因表达
liver neoplasms
carcinoma, hepatocellular
transforming growth factor β1
gene expression
RNA, messenger
