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应用光镊子拉曼光谱鉴别阴道毛滴虫和口腔毛滴虫的研究 被引量:2

Identification of Trichomonas Vaginalis and Trichomonas Tenax Using Laser Tweezers Raman Spectroscopy
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摘要 应用激光镊子拉曼光谱技术比较分析阴道毛滴虫和口腔毛滴虫的拉曼吸收峰,寻找具有分类学鉴别价值的特征拉曼峰。通过光镊随机俘获单个大小一致、有活力的虫体,记录其拉曼光谱,收集数据并做主成分分析。在937cm-1、1002cm-1和1446cm-1谱峰的强度和谱型上有差异,根据1002cm-1峰的信号强度,并结合937cm-1和1002cm-1峰强度比值(I937/I1002)以及1002cm-1和1446cm-1峰强度比值(I1002/I1446)可以作为区分阴道毛滴虫和口腔毛滴虫的量化指标。该法是简便、快捷、有效地鉴别两种人体毛滴虫的新方法。 Laser tweezers Raman spectroscopy was used to identify Trichomonas vaginalis(TV) and Trichomonas tenax(TT).The Raman spectra of 30 trichomonads were randomly acquired,in which 20 polypides were randomly used to carry out a principal components analysis(PCA).The mean Raman spectra of TV among their strains were as well as TT,but the Raman bands at 1002cm-1 of TV showed significant differences compared to TT and the intensity of TV at 1002cm-1 peak is obviously higher around 2 folds than TT.The ratio I937/I1002 of TV strains was lower than those of TT strains,on the contrary,the ratio I1002/I1446 of TV strains was higher about 2 folds than TT strain's.Above values can be used as an index quantificationally to identify TV and TT.The method can be used to rapidly discriminate trichomonads from various species.
出处 《光谱实验室》 CAS CSCD 北大核心 2010年第3期1143-1147,共5页 Chinese Journal of Spectroscopy Laboratory
基金 广西自然科学基金项目(No.0728177) 广西中医学院自然科学研究基金(P2008099)资助
关键词 光镊子 拉曼光谱 阴道毛滴虫 口腔毛滴虫 Laser Tweezers Raman Spectroscopy Trichomonas vaginalis Trichomonas tenax
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参考文献11

  • 1徐敏,尹跃平,余艳华,刘爱英,王书崎.PCR在阴道毛滴虫检测中的引物筛选和条件优化[J].中华皮肤科杂志,2004,37(10):603-605. 被引量:2
  • 2Buijtels P C A M, Willemse-Erix H F M,Petit P L C et al. Rapid Identification of Mycobacteria by Raman Spectroseopy[J]. Journal of Clinical Microbiology,2008,46(3) :961-965. 被引量:1
  • 3Maquelin K L, Dijkshoorn T J, Van der Reijden T Jet al. Rapid Epidemiological Analysis of Acinetobacter Strains by Raman Spectroscopy[J].J. Microbiol. Methods,2006,64 (1) :126-131. 被引量:1
  • 4Xie C,Mace J,Dinno M A et al. Identification of Single Bacterial Cells in Aqueous Solution Using Confocal Laser Tweezers Raman Spectroscopy[J]. Anal. Chem. ,2005,77(14),4390 -4397. 被引量:1
  • 5Chen D,Huang S S, Li Y Q. Real-Time Detection of Kinetic Germination and Heterogeneity of Single Bacillus Spores by Laser Tweezers Raman Spectroscopy[J]. Analytical Chemistry,2006,78(19):6936-6941. 被引量:1
  • 6Bellanger A P,Cabaret O,Costa J M et al. Two Unusual Occurrences of Trichomoniasis:Rapid Species Identification by PCR[J]. J. Clin. Microbiol. , 2008,46(9) : 3159-3161. 被引量:1
  • 7陈秀丽,王桂文,刘军贤,陈萍,黎永青.基于拉曼光谱的地贫红细胞种类识别方法的研究[J].分析测试学报,2009,28(4):403-408. 被引量:7
  • 8王桂文,姚辉璐,何碧娟,彭立新,黎永青.单个血小板的拉曼光谱分析[J].光谱学与光谱分析,2007,27(7):1347-1350. 被引量:16
  • 9姚辉璐,朱淼,王桂文,彭立新,何碧娟,何敏,黎永青.单个鼻咽癌细胞的拉曼光谱分析的研究[J].光谱学与光谱分析,2007,27(9):1761-1764. 被引量:20
  • 10许以明编著..拉曼光谱及其在结构生物学中的应用[M].北京:化学工业出版社,2005:151.

二级参考文献56

  • 1闫循领,董瑞新,王秋国,陈双峰,张宗旺,张学俊,张雷.乳腺癌病人单个细胞的Raman光谱[J].光谱学与光谱分析,2005,25(1):58-61. 被引量:25
  • 2KELLER S, SCHRADER B, HOFFMANN A, et al. Application of near-infrared - Fourier transform Raman spectroscopy in medical research[ J]. J Raman Spectrosc, 1994, 25:663 -667. 被引量:1
  • 3XIE Changan, LI Yongqing. Raman spectra and optical trapping of highly refractive and nontransparent particles[ J]. Appl Phys Lett, 2002, 81(6) : 951 -953. 被引量:1
  • 4XIE Changan, DINNO M A, LI Yongqing. Near-infrared Raman spectroscopy of single optically trapped biological cells [ J ]. Opt Lett, 2002, 27 (4) : 249 - 251. 被引量:1
  • 5RAMSER K, LOGG K, GOKSOR M, et al. Resonance Raman spectroscopy of optically trapped functional erythrocytes [J]. J Biomed Opt , 2004, 9(3): 593-600. 被引量:1
  • 6RAMSER K, LOGG K, ENGER J, et al. Resonance Raman study of the oxygenation cycle of optically trapped single red blood cells in a microfluidic system[ J]. Proc SPIE, 2004, 5514:560 -568. 被引量:1
  • 7SANDERSON J M, WARD A D. Analysis of liposomal membrane composition using Raman tweezers[ J]. Chem Commun (Cambridge) , 2004, 7 : 1120 - 1121. 被引量:1
  • 8XIE Changan, LI Yongqing, TANG Wei, et al. Study of dynamical process of heat denaturation in optically trapped single microorganisms by near-infrared Raman spectroscopy[ J ]. J Appl Phys, 2003, 94 (9) : 6138 -6142. 被引量:1
  • 9XIE Changan, GOODMAN C, DINNO M A, et al. Real-time Raman spectroscopy of optically trapped living cells and organelles [ J ]. Opt Express, 2004, 12 (25) : 6208 - 6214. 被引量:1
  • 10CHAN J W, ESPOSITO A P, TALLEY C E, et al. Reagentless identification of single bacterial spores in aqueous solution by confocal laser tweezers Raman spectroscopy[ J ]. Anal Chem, 2004, 76:599 -603. 被引量:1

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