摘要
目的:比较上皮性钙黏蛋白(E-cadherin,E-cad)在人输卵管妊娠种植与非种植部位组织中的表达水平。方法:14例未破裂型输卵管壶腹部妊娠组织标本,均包括种植和非种植部位的组织,分为种植部位组(n=14)和非种植部位组(n=14);并以12例因其它疾患行全子宫加附件切除术的人分泌期正常输卵管壶腹部组织新鲜标本为对照组。采用免疫组织化学法观察E-cad蛋白在输卵管妊娠组织和输卵管黏膜上皮的表达情况,应用荧光定量聚合酶链反应(qRT-PCR)和免疫印迹法(Western blotting)检测其E-cadmRNA和蛋白表达水平。结果:E-cad蛋白表达于人输卵管黏膜上皮纤毛细胞和分泌细胞的胞膜,在细胞之间的连接面表达较强,管腔面表达较弱,纤毛未见表达。在输卵管妊娠组织,E-cad表达于蜕膜细胞、绒毛细胞滋养层细胞和绒毛外滋养细胞的胞膜,绒毛外滋养细胞的阳性显色弱于绒毛细胞滋养层细胞。E-cadmRNA和蛋白在种植部位组的表达显著低于非种植部位组和对照组(P<0.05),非种植部位组与对照组之间表达无显著性差异(P>0.05)。结论:E-cad在输卵管妊娠种植部位表达水平降低,可能与输卵管妊娠发生有密切的联系。
Objective： To compare expressions of E-cadherin （E-cad） between the implantation and the non-implantation sites for tubal ectopic pregnant tissues. Methods： Samples of tubal ectopic pregnant tissues （n=14） were divided into the implantation site group （n=14） and the non-implantation site group （n=14）. Samples of oviductal ampullae tissues in the secretory stage as the control （n=12） were obtained from women undergoing abdominal hysterectomy with adnexectomy for benign disease such as uterine leiomyoma, adenomyosis in the pelvic cavity. Expressions of E-cad were determined by the immunohistochemical, real-time fluoregenic quantitative polymerase chain reaction （qRT-PCR）, and Western blotting methods in the three groups. Results： Positive staining of E-cad was observed in the membrane of the epithelial cells in the non-implantation site and oviductal ampullae tissues. In addition, positive staining was also shown in the membrane of decidual cells, cytotrophoblast, and extravillous trophoblast in the implantation site. Expressions of E-cad mRNA and protein in the implantation site group had lower levels than those of the non-implantation site group or the control （P0.05）. Expressions of E-cad mRNA and protein between the non-implantation site group and control group showed no significant difference （P0.05）. Conclusion： The implantation site of tubal pregnant tissues would have lower E-cad level, which could be involved in aetiology of tubal pregnancy.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2010年第4期240-244,共5页
Reproduction and Contraception
