摘要
In previous study we reported that pretreatment with plasmolysis enhanced somatic embryo formation in hypocotyls of Eleutherococcus senticosus.In the present study,the expression level of callose synthase gene in embryos of E.senticosus in response to 2,4-D,sucrose and mannitol treatments was analyzed by RT-PCR.The results show that plasmolysis pretreatment using sucrose and mannitol significantly promoted the expression of callose synthase gene.Also,the thicker cell walls of explant plasmolyzed compared with controls were observed during the somatic embryogenesis.We suggest that the callose may make the cells in epidermis separate from neighboring cells and then develop into embryogenic potential cells.
在以前的研究中我们报道了经质壁分离预处理刺五加合子胚下胚轴,促进了体细胞胚形成。在本研究中,我们利用RT-PCR方法分析了刺五加合子胚经2,4-D,蔗糖,甘露醇处理后胼胝质合成酶基因的表达水平。结果显示:蔗糖和甘露醇处理明显促进了胼胝质合成酶基因的表达水平。而且我们观察到,体细胞胚发生过程中,外植体经质壁分离处理后细胞壁明显加厚。因此我们推测:胼胝质可能使表皮层细胞相互之间产生分离从而使其转变为具有胚性潜能的细胞。图2参20。
基金
supported by the 948 Program of State Forestry Administration (No2009-4-26)
co-sponsored by SRF for ROCS and the National Natural Science Foundation (No 30972390) of China
