摘要
目的:探讨荧光定量PCR技术在筛查甲型流感病毒中的诊断价值。方法:用荧光定量RT-PCR方法检测150例疑似流感患者咽拭子中的甲型流感病毒RNA,用XT-1800全自动血液分析仪检测150例疑似流感患者EDTA抗凝全血中的白细胞计数,用胶体金法检测54例甲型流感病毒RNA阳性患者的甲型流感病毒核心蛋白。结果:在150例患者中,甲型流感病毒RNA检测阳性者有54例,阳性率为36%。54例甲型流感病毒RNA检测阳性者采用胶体金法检测甲型流感病毒核心蛋白,结果均为阴性。甲型流感病毒RNA检测阳性者白细胞计数为(6.81±2.12)×109/L,阴性者为(6.64±3.13)×109/L,白细胞计数在甲型流感患者和非甲型流感患者中无统计学差异(P>0.05)。结论:荧光定量RT-PCR在检测甲型流感病毒RNA中有较好的阳性检出率,其敏感性和特异性明显优于胶体金方法和白细胞计数,能快速有效筛选出甲型流感患者,防止疫情爆发流行。
AIM：To discuss the the diagnostic value of fluorescence quantitative PCR in screening of influenza A virus. METHODS：Influenza A virus RNA in 150 cases of suspected influenza patients with throat swab was measured by fluorescence quantitative RT-PCR. The white blood cell count was measured by XT-1800 automated hematology analyzer in patients＇ anticoagulant EDTA whole blood. 45 cases of influenza A virus RNA-positive patients were detected with influenza A virus core protein by colloidal gold. RESULTS：In 150 patients,54 cases were detected influenza A virus RNA positive,the positive rate was 36%.The 54 cases of influenza A virus RNA-positive patients were detected with influenza A virus core protein by colloidal gold,the results were all negative.The white blood cell count is （6.81±2.12）×10^9/L in the influenza A virus RNA-positive patients,and （6.64±3.13）×10^9/L for the negative cases. White blood cell count in patients with influenza A and non-influenza patients have no significant difference. CONCLUSION：Fluorescence quantitative RT-PCR in the detection of influenza A virus RNA has a better positive rate,its sensitivity and specificity are better than colloidal gold and white blood cell count,and it can be screened quickly and efficiently in patients with influenza to prevent disease outbreaks.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2010年第4期356-358,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
全军"十一五"计划资助(08Z009)
关键词
甲型流感病毒
荧光定量PCR
胶体金
白细胞计数
influenza A virus
fluorescence quantitative PCR
colloidal gold
white blood cell count
