摘要
Objective： To elucidate protein markers that differentiate stage I non-small cell lung cancer （NSCLC） that subsequently develop metastatic disease to those that do not develop metastasis by protein expression profiles. Methods： Matrix assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF MS） and two dimensional difference gel electrophoresis （2D-DIGE） platforms were used to separate proteins in whole tumor specimens. Quantitating mRNA expression was used for validation. Results： Twelve proteins were identified as expressed differentially between two groups from protein expression platforms. But from gene expression platform no marker could distinguish patients with recurrent vs. nonrecurrent disease. Conclusion： Analysis of multiple protein markers may be more informative to predict prognosis of early stage lung cancer.
Objective： To elucidate protein markers that differentiate stage I non-small cell lung cancer （NSCLC） that subsequently develop metastatic disease to those that do not develop metastasis by protein expression profiles. Methods： Matrix assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF MS） and two dimensional difference gel electrophoresis （2D-DIGE） platforms were used to separate proteins in whole tumor specimens. Quantitating mRNA expression was used for validation. Results： Twelve proteins were identified as expressed differentially between two groups from protein expression platforms. But from gene expression platform no marker could distinguish patients with recurrent vs. nonrecurrent disease. Conclusion： Analysis of multiple protein markers may be more informative to predict prognosis of early stage lung cancer.
基金
supported by NIH grant (1RO1-CA-109384-01A)
