摘要
STRA8是哺乳动物生殖细胞由有丝分裂转变为减数分裂前特异表达的基因。STRA8在出生后至成年仅在睾丸减数分裂前的精原细胞上表达,一部分性腺发育不全患者发现有STRA8基因单核苷酸多态性变化,但未发现STRA8基因编码区和近端启动子区有突变。用STRA8基因启动子序列构建调控表达增强型绿色荧光蛋白STRA8-EGFP的载体转染畸胎瘤细胞、胚胎干细胞的研究表明,STRA8-EGFP阳性细胞能经减数分裂发育成精子并生出子代小鼠,骨髓来源的EGFP-STRA8阳性细胞能分化为生精细胞,但分化停滞在减数分裂前期阶段;成年转基因小鼠STRA8-EGFP阳性精原干细胞,在胚胎干细胞(ESC)培养条件下培养,具有ESC的表达特征,具有多能性,可再分化,为组织再生提供了一个新的干细胞来源;出生后卵巢存在新生卵母细胞,STRA8在出生后雌性生殖细胞不再表达的论断受到质疑。
Stimulated by retinoic acid gene 8（STRA8） is specifically expressed in mammalian germ cells before their transition from mitosis to meiosis.STRA8 expression is observed only in the postnatal testis.Single nucleotide polymorphisms but no mutations were identified in the coding or proximal promoter region of STRA8 in some gonadal dysgenesis patients.Studies on the teratocarcinoma cells and embryonic stem cells（ESC） transfected with STRA8-EGFP,a fusion construct harboring the promoter and coding region of the enhanced green fluorescence protein,have shown that the STRA8-EGFP positive cells may undergo meiosis,develop into sperm and generate live offspring mice.STRA8-EGFP positive cells derived from the bone marrow are able to differentiate into spermatogenic cells,but arrest in the premeiotic stage,and those from the adult mouse testis,when cultured in ESC culture conditions,may acquire ESC properties,pluripotency and redifferentiation capacity and act as a new stem cell source for tissue regeneration.The presence of oocytes renewed in postnatal mouse ovaries calls in question the absence of STRA8 in postnatal mouse ovaries.
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2010年第2期161-165,共5页
National Journal of Andrology
基金
国家自然科学基金(30360032
30960409)
江西省教育厅科研项目(GJJ09103)~~
