摘要
探讨丹参骨宝对维甲酸模型大鼠股骨生物力学及骨密度的影响。4月龄未交配的SPF级雌性SD大鼠40只,随机分成5组(每组8只):正常对照(CON)组、维甲酸模型(RA)组、丹参骨宝高剂量(DSH)组、丹参骨宝中剂量(DSM)组、丹参骨宝低剂量(DSL)组。CON组给予溶剂对照溶液,其它4组上午先用维甲酸70 mg.kg-1.d-1造模,然后同时下午给予不同药物预防,RA组给予溶剂对照溶液,DSH组、DSM组和DSL组分别给予含丹参素10、5和2.5 mg.kg-1.d-1的丹参骨宝。大鼠每次均按5 ml.kg-1灌胃给药,连续给药28 d。所有大鼠在处死前的第13、14 d和第3、4 d分别皮下注射盐酸四环素30 mg.kg-1和钙黄绿素5 mg.kg-1进行荧光标记。大鼠于实验结束时处死取材,取右侧股骨进行骨物理指标、骨密度和骨生物力学参数的测量。结果发现与CON组相比,RA组大鼠股骨物理指标(横断面的外径、湿重和干重)降低(P<0.05),股骨中点和远心端的骨密度均下降(P<0.05),股骨生物力学指标(最大载荷、弹性载荷、断裂应变、刚性系数、弯曲能量)均下降(P<0.05)。与RA组比较,DSH组和DSM组大鼠的股骨远心端的骨密度均增加(P<0.05),但其它指标无明显变化。本研究表明,维甲酸致大鼠股骨物理指标、骨密度和生物力学参数均下降,丹参骨宝可对抗维甲酸致大鼠股骨骨密度的降低,但对物理指标和生物力学参数无明显影响。
This investigation was directed to the effects of DanShenGuBao on biomechanical properties and bone mineral density（BMD） of femur induced by retinoic acid（RA） in rats.Forty 4-month-old virgin female Sprague-Dawley rats were randomly divided into 5 groups（8 rats each）： control group,RA group and different doses of DanShenGuBao groups.Rats in control group were given vehicle,rats in other four groups were given RA at 70mg·kg-1·d-1 in the morning and given different drugs in the afternoon at the same time.Rats in RA group were given vehicle,rats in other groups were given different doses of DanShenGuBao which contained 10mg·kg-1·d-1,5mg·kg-1·d-1,2.5mg·kg-1·d-1 tanshinol,respectively.All of rats were treated at 5ml·kg-1 by oral gavaged for 28 days.In preparation for the determination of dynamic changes in bone tissues,all rats were given subcutaneous injections of 30mg·kg-1 tetracycline on the 14th,13th day and 5mg·kg-1 calcein on the 4th,3rd day before death.At the experimental endpoint,the rats were sacrificed by cardiac puncture under sodium pentobarbital anesthesia.Physical parameters,BMD and biomechanical properties of femur were assessed.Compared with those in control group,the physical parameters （cross-sectional diameter,wet and dry weight）,BMD and biomechanical properties（max-load,elasticity-load,break-strain,rigid coefficient and bending-energe） were significantly decreased in RA group.Compared with that in RA group,the BMD of femur was increased significantly in medium and high dose of DanShenGuBao group,but there was no significant change in physical parameters and biomechanical properties of femur.RA could decrease the physical parameters,BMD and biomechanical properties of femur.DanShenGuBao could increase BMD,but it was found with no obvious effect on physical parameters and biomechanical properties.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
2010年第2期307-310,共4页
Journal of Biomedical Engineering
基金
国家自然科学基金资助项目(30672470)
关键词
丹参
维甲酸
生物力学
骨密度
大鼠
Danshen
Retinoic acid
Biomechanical properties
Bone mineral density（BMD）
Rat
