摘要
采集河南省规模化猪场流产母猪死胎脑脊液作为样本,通过细胞盲传培养分离乙型脑炎病毒流行毒株,对新分离的病毒进行分子生物学鉴定,获得1株分离株并命名为CSF.ZMD-3。DNA序列测定及比对分析该分离株与已报道毒株基因的序列同源性,确定该分离毒株为乙脑病毒。使用MEGA3.1软件对CSF.ZMD-3毒株主要结构蛋白E蛋白的基因序列进行分子进化分析,确定CSF.ZMD-3为基因Ⅲ型乙脑病毒。
In this study,cerebrospinal fluid （CSF） of sow aborted fetus stillbirth were collected from large pig farms in Henan province,and an isolate named as CSF. ZMD- 3 was obtained by inoculating PK - 15 cells and identified by RT - PCR. By comparing the E gene sequence of CSF. ZMD-3 with those of previously reported JEV strains, we are sure that this isolate is a JEV strain. Using the E genes of CSF. ZMD- 3 and other 18 reference strains,a phylogenetie tree was constructed by Mega 3.1 software and showed that CSF. ZMD - 3 belongs to gene type III.
出处
《河南农业科学》
CSCD
北大核心
2010年第4期101-104,共4页
Journal of Henan Agricultural Sciences
基金
农业部公益性行业科研专项(200803015)
河南省公益性行业科研专项(2008-145)
关键词
乙型脑炎病毒
分离鉴定
M基因
E基因
基因进化树
Japanese encephalitis virus
Isolation and identification
M gene
E gene
Phylogenetic tree
