摘要
目的探讨抗亚洲带绦虫药物对于重组的亚洲带绦虫乳酸脱氢酶(Ta.LDH)酶促功能的影响。方法将不同浓度的吡喹酮、阿苯达唑和甲苯咪唑加入Ta.LDH所催化的丙酮酸还原成乳酸(正反应)以及乳酸氧化成丙酮酸(逆反应)的标准反应体系当中,测定烔?废汆堰?核苷酸(NADH)在A340处吸光度的变化值。采用SPSS 16.0软件进行分析,计算药物对酶活性的相对抑制率。结果与对照组比较,0.10 mmol/L吡喹酮对Ta.LDH催化正逆反应的相对抑制率分别为93.99%(P<0.01)和94.67%(P<0.01),0.10 mmol/L阿苯达唑分别为85.45%(P<0.01)和73.81%(P<0.01);0.15 mmol/L甲苯咪唑分别为92.2%(P<0.01)和86.13%(P<0.01)。结论Ta.LDH可能为吡喹酮、阿苯达唑和甲苯咪唑抗亚洲带绦虫药物作用的靶标分子。
Objective To detect the effect of praziquantel,albendazole,and mebendazole on activity of the recombinant lactate dehydrogenase of Taenia asiatica（rTaLDH）.Methods The effects of praziquantel,albendazole,and mebendazole on the enzymatic activity of rTaLDH were detected by spectrophotometric method in standard reaction system established before.The solvent of each reagent was used as control.The results were analyzed with SPSS16.0 software.Results Compared with the control,considerable inhibition for both reduction and oxidation reactions catalyzed by rTaLDH was observed at 0.10mmol/L of praziquantel（P0.01）.Compared with the control,the enzyme activities of rTaLDH for reduction and oxidation reactions were inhibited by 93.99% and 94.67%,respectively,at 0.1 mmol/L of praziquantel（P0.01）.At 0.15mmol/L of mebendazole,enzymatic activities were inhibited by 92.2% for reduction reaction and 86.13% for oxidation reaction,respectively,lower than that of the control（P0.01）.Conclusion Praziquantel shows a high inhibition on rTaLDH.TaLDH may be one of the molecular targets of praziquantel,albendazole,and mebendazole.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2010年第5期553-554,共2页
Chinese Journal of Public Health
基金
国家自然科学基金(30760227)
贵州省科技攻关项目(2008-3060)
贵州省农业科技攻关项目〔黔科合NY字(2009-3074)〕
贵州省科技攻关项目(2009-3101)
贵州省省长基金〔黔省专合字(2009)82号〕
贵阳市科技局社发攻关项目(2009-3005)
关键词
亚洲带绦虫
乳酸脱氢酶
重组蛋白
吡喹酮
阿苯达唑
甲苯咪唑
Taenia asiatica
lactate dehydrogenase
recombinant protein
praziquantel
albendazole
mebendazole
