摘要
研究了稳定表达hERG基因的CHO-K1(中国仓鼠卵巢细胞)细胞株及其在药物研发安全性筛选方面的应用。使用Lipofectamine 2000系统将hERG基因转染入CHO-K1细胞,用Western Blot检测其表达,并进一步通过FluxORTM Thallium Assay Kits验证;同时使用FluxORTM Thallium Assay Kits检测Cisapride和Dofetilide对hERG通道的抑制性。建立了4株稳定表达hERG基因的CHO-K1细胞株,Cisapride和Dofetilide对hERG通道的IC50分别为86.7 nmol.L-1和28.1 nmol.L-1。hERG基因成功地在CHO-K1细胞株实现了稳定表达,此细胞株可用于FluxORTM Thallium Assay Kits系统,用于药物研发安全性筛选,以评估化合物潜在的心脏毒性。
CHO-K1 （Chinese hamster ovary cell）cell lines stably expressing hERG gene and its application in drug R＆D safety screening were studied, hERG gene was transfected into CHO-K1 cell with Lipofectamine 2000, and the expression of hREG was detected by using Western Blot technology and FluxORTM Thallium Assay Kits, proving the inhibition of bERG by detec- ting Cisapride and Dofetilide. Four cell lines stably expressing hERG gene was established, and ICTM are, respectively, 86. 7 nmol · L^-1, 28.1 nmol · L^-1 of Cisapride and Dofetilide. hERG is transfected into CHO-K1 cell lines successfully, and the cell lines can be used to drug R＆D safety screening in FluxORTM Thallium Assay Kits.
出处
《河北科技大学学报》
CAS
北大核心
2010年第2期137-141,共5页
Journal of Hebei University of Science and Technology
