摘要
目的：观测Dact1基因在结直肠癌组织的表达及其启动子甲基化状态,并探讨它们之间的关系及在结直肠癌发生中的作用.方法：采用逆转录聚合酶链反应（RT-PCR）检测结直肠癌组织、癌旁组织各50例及正常对照组织20例Dact1基因mRNA的表达情况,甲基化特异性PCR（MSP）检测Dact1基因启动子甲基化状态.结果：结直肠癌组中,9例Dact1基因mRNA失表达（18%）,20例表达低于正常对照组（40%）;癌旁组织组中,1例Dact1基因mRNA失表达（2%）,3例表达低于正常对照组（6%）;正常对照组中,1例Dact1基因mRNA低表达（5%）.Dact1基因在癌组织、癌旁组织、正常对照组织中的甲基化阳性率分别为46%、12%、5%,癌组织组与癌旁组织组及正常对照组阳性率比较,差异均有统计学意义（P＜0.01）,而癌旁组织组和正常对照组阳性率比较,差异无统计学意义（P＞0.05）.Dact1基因mRNA的失表达（低表达）与其启动子甲基化状态的相关性分析有统计学意义（P＜0.05）.结论：Dact1基因mRNA的失表达（低表达）可能参与了结直肠癌的发生发展,其失表达可能与Dact1启动子区甲基化相关.
Objective：To observe the methylation of Dactl promoter and expression of Dactl mRNA in colorectal cancer, further to analyze their correlation and explore the role of gene Dactl in the eoloreetal carcinogenesis. Methods：Reverse transcription PCR（RT-PCR） technique was used to detect the expression of Dactl mRNA in each 50 specimens of colorectal tumor tissues and their adjacent tissues and 20 normal tissues. The methylated condition of Dactl promoter was evaluated by employing the methylation-specific PCR（MSP） assay. Results：The expression of Dactl mRNA was silenced and down-regulated in 9 （ 18% ） and 20 （40%） speci- mens of colorectal cancer tissues, in 1 （2%） and 3 （6%）adjacent tissues, in 0（0% ）and 1 （5%）normal tis- sue, respectively. The positive rates of methylation of Dactl promoter in the corresponding tissues were 46% , 12% and 5% , respectively. The positive rate in colorectal cancer tissues was highest and has statistical significance （ P 〈 0.01 ） compared with the other two groups between which no statistical difference （ P 〉 0.05 ）. There correlation analysis of the silencing （and down-regulation） of Dactl gene and the methylated condition of Dactl promoter displayed a statistical significance（ P 〈 0.05 ）. Conclusions：The silencing （and down-regulation） of Dactl mRNA maybe participate in the colorectal carcinogenesis, and is related to the methylation of Dactl promoter.
出处
《解剖与临床》
2010年第2期105-108,共4页
Anatomy and Clinics
基金
杭州市医学重点专科专病项目（编号20080333Q07）
