摘要
为了优化将抗体偶联在二氧化硅试管表面上以便进行丙型肝炎抗原检测的分析系统,本研究通过氨基硅烷的活化作用,在玻璃表面形成活化的氨基,以戊二醛作为化学交联试剂,在已硅化的玻璃表面固定丙肝单克隆抗体,并进行丙肝抗原(HCAg)的测定。结果显示,通过条件优化实验,发现以10%(V/V)的氨基硅烷水溶液处理玻璃试管3h后,再用3%(V/V)的戊二醛水溶液交联丙肝单克隆抗体2h,可以得到固定效果较好,非特异性较低的玻璃载体,对HCAg可测至1μg/L。可见,用该方法制备的玻璃载体可为进一步建立新的HCAg磁性免疫检测系统提供理论和实验依据。
In order to optimize the fabrication of SiO2 tubes immobilized with antibody for hepatitis C virus antigen （HCAg） detection, we formed the activated amino on the surface of SiO2 tubes by using the activation of aminosilane. Then we immobilized the hepatitis C virus （HCV） monoclonal antibody on the surface of SiO2 tubes by using glutaraldehyde as a chemical cross-linker, followed by detecting HCAg. Sequence tests showed that when the SiO2 tubes were treated in 10% （V/V） aminosilane solution and 3% （V/V） glutaraldehyde solution for 3 hours and 2 hours, respectively, the HCV monoclonal antibody had high immobilization efficiency and low nonspecificity, and the HCAg was detected to 1 ng/mL. This experiment can provide principle and experimental data for establishment of HCAg magnetic immunoassay system.
出处
《生物工程学报》
CAS
CSCD
北大核心
2010年第4期545-549,共5页
Chinese Journal of Biotechnology
基金
国家高技术研究发展计划(863计划)(No.2007AA03Z315)
首都医学发展科研基金(No.2007-3080)资助~~
关键词
丙肝病毒抗原
丙肝单克隆抗体
共价交联
氨基硅烷
戊二醛
SiO2载体
hepatitis C virus antigen
HCV monoclonal antibody
covalent immobilization
aminosilane
glutaraldehyde
SiO2 tubes
