摘要
目的分析HES1在弱精症患者低活力精子中的表达及调控。方法收集30份弱精症患者精液标本和20份成年健康有生育能力男性的精液标本,提取精子RNA,制备生物素标记的cDNA探针,与含有30 968个探针的Phal-anx OneArrayTM芯片杂交,分析男性精子中HES1基因的表达情况;再用RT-PCR,分析hsa-miR-487a和hsa-miR-193b在精子中的表达,从精子mRNA表达谱中搜索hsa-miR-487a和hsa-miR-193b靶基因的表达。结果HES1在低活力精子中的表达上调,高于在高活力精子中的表达。hsa-miR-487a和hsa-miR-193b在低活力精子中的表达量分别是高活力精子的0.43倍和2.19倍。结论HES1在低活力精子中的表达上调,可能通过hsa-miR-487a和hsa-miR-193b的调节来影响精子的活力。
Objective To analyze the expression and regulation of HES1 in sperms with low motility.MethodsThirty semen samples from asthenospermia patients and 20 semen samples from healthy and fertile adults were collected,total RNAs were extracted to produce cDNAs probes.Hybridization with Phalanx OneArrayTM containing 30 968 probes was carried out after the labeled cDNAs were purified by PCR product purification kit.Realtime RT-PCR was used to analyze the expression of hsa-miR-487a and hsa-miR-193b;the expression of the target genes of hsa-miR-487a and hsa-miR-193b were searched from gene-expression profiles in asthenospermia patients＇ sperms.Results The expression level of HES1 in low motility sperms was up-regulated.The expression level of hsa-miR-193b in low motility sperms was 2.19 times higher than that in high motility sperms,hsa-miR-487a was 0.43% of that in high motility sperms.Conclusion The expression level of HES1 in low motility sperms was up-regulated.Hsa-miR-487a and hsa-miR-193b may affect the expression of HES1 and so regulate sperm motility.
出处
《基础医学与临床》
CSCD
北大核心
2010年第5期505-509,共5页
Basic and Clinical Medicine
基金
广东省自然科学基金(81515031020000027)
广东省科技计划项目(2008B03031242)
