摘要
[目的]进一步了解杜仲叶降血脂、抗氧化作用的活性,以期为杜仲的综合开发利用提供依据。[方法]将ICR小鼠按体重随机分为正常对照组和高脂饲料组,正常对照组动物从试验开始至结束用普通饲料喂养,高脂饲料组动物均喂高脂饲料,制造成营养性高脂血症模型供试小鼠。再将高脂饲料组动物按体重随机分为模型对照组,阳性药对照组,黄酮大、中、小剂量组,动物继续给予高脂饲料,模型对照组每天给予相应体积的溶媒,黄酮大、中、小3个剂量组,分别以45、15和5mg/(kg.d)剂量的黄酮灌胃给药,阳性药组给予5mg/(kg.d)的洛伐他汀(相当于人用剂量按照标准体表面积折算后小鼠用剂量的2倍),正常对照组给以等体积溶媒。17d后,各组小鼠均禁食12h,眼眶取血,肝素抗凝,各取0.2ml血液溶血后用于CAT测定,其余抗凝血液离心分离血浆用于血脂及POD、MDA的测定。[结果]与高脂血症模型组相比较,杜仲叶黄酮给药组动物的血浆总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、动脉粥样硬化指数(AI)均有不同程度的降低,高密度脂蛋白胆固醇(HDL-C)有所上升,血中过氧化氢酶(CAT)、过氧化物酶(POD)活性增强,血浆中丙二醛(MDA)的含量下降。[结论]杜仲叶黄酮具有降低营养性高脂血症小鼠血脂和抗氧化的作用。
[ Objective] The paper aimed to further observe the activity of reducing blood lipid and antioxidition effects of flavonoids from Eucommia ulmiodes Olirer Leaves.[Method] ICR mice were randomly divided into the normal control group and high-fat diet group, the normal control group was fed by common feedstuffs, while high-fat diet group fed by high-fat diet, which made into tested mice of a nutrition, hyperlipidemia model. And high-fat diet group were randomly divided into model control group, positive medicine control group, flavonoids large, medium and small-dose groups, animals were fed by high-fat diet, model control group was offered by corresponding volume of solvent, large, medium and small-dose groups were taken medicine by flavonoids gavage with 45 ,15 and 5 mg/（kg·d） dose, positive drug group was offered by Lovastatin with 5 mg/（kg·d） （equivalent to human dose conversion according to the standard body surface area of mice with 2 times doses） , the normal control group was offered by equal volume of solvent. After 17 d, each group of mice was fasted by 12 h, orbital blood, heparin, 0.2 ml of blood from each of hemolysis for the CAT after the determination of the rest of the blood and anticoagulant used for centrifugal separation of plasma lipids and POD, MDA determination. [Result] Compared with hyperlipidemia model group, TC,TG, LDL-C and AI flavonoids from Eucommia ulmiodes Olirer Leaves had degraded with different degrees, HDL-C had raised, activity of CAT,POD had strengthened, content of MDA in plasma had degraded. [Conclusion] Flavonoids from Eucommia ulmiodes Olirer Leaves had effects of reducing the nutritional hyperlipemia in mice and anti-lipid oxidation.
出处
《安徽农业科学》
CAS
北大核心
2010年第11期5631-5632,共2页
Journal of Anhui Agricultural Sciences
关键词
杜仲叶
黄酮
高脂血症
抗氧化
Eucommia ulmiodes Olirer Leaves
Flavonoids
Hyperalimentation
Antioxidition
