摘要
[目的]研究放线菌快速检测方法的PCR反应体系及反应条件的优化。[方法]以5种存在于16SrDNA序列中的放线菌特异性的特征序列为引物,通过对PCR反应体系和反应条件的优化,建立最佳扩增体系及反应条件,并对供试菌株进行测定,以验证该方法的可靠性。[结果]所采用的特异性引物能够有效检测出特定类群的放线菌,从而达到快速检测不同类群放线菌的目的。[结论]该方法可用于放线菌的快速检测,为放线菌的分类鉴定研究提供参考。
[Objective] The aim was to optimize the method and system on rapid detection of actinomycetes.[Method] Using five kinds of characteristic sequences existed in the 16S rDNA sequence of Actinomycetes as five pairs of primers,the optimal amplification conditions were established.The tested strains were determined to verify the reliability of the method.[Result] The results showed that the designed primers could be used to detect the designated actinomycetes effectively,so as to achieve the purpose of rapid detection of different actinomycetes.[Conclusion] The method could be applied in rapid detection of actinomycetes,which provided a reference for classification and identification of actinomycetes.
出处
《安徽农业科学》
CAS
北大核心
2010年第11期5539-5541,共3页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金:共生固氮放线菌分类系统的研究(30770003)
河北省微生物资源基础信息数据库(0996-7119D-16)
关键词
放线菌
特异性引物
快速检测
Actinomycetes
Specific primers
Rapid detection
