摘要
目的 测定63例缺铁性贫血(IDA)合并反复呼吸道感染患儿外周血白细胞介素2(IL-2)活性、血清可溶性白介素2受体(sIL-2R)以及T淋巴细胞亚群,并分析他们之间的相关性.方法 T淋巴亚群测定采用APAAP法,IL-2活性测定采用MTT比色法,sIL-2R测定采用单抗与多抗双抗体夹心法.结果 IL-2活性、CD_3、CD_4、CD_4/CD_8显著低于对照组(P<0.01),而sIL-2R水平显著高于对照组(P<0.01),CD_8无明显变化.结论 IDA合并RRI患儿细胞免疫功能低下且紊乱,为临床应用免疫治疗提供依据.
ve To determine IL-2 activity, sIL-2R, T lymphocyte subsets in peripheral blood of 63 children patients suffered IDA with RRI. Methods IL-2 activity, sIL-2R and T lypho-cyte were detetrmined by MTT colorimetric assay, Sandwich ELISA with monoclonal and polyclonal antibodies as well as APAAP assay. Results IL-2 activity, the percent of CD3 and CD4 as well as the ratio of CD4/CD8 were significantly lower than those of control group (P<0. 01), While sIL-2R was higher. Conclusion Cellular immunity was deficient and disorder in patients who were indicated for adoptive immunotherapy
关键词
缺铁性贫血
呼吸道感染
儿童
细胞免疫
IDA Recurrent respiratory infection IL-2 sIL-2R T lymphocyte
