摘要
目的探讨不同的方法对人骨髓间充质干细胞向成骨细胞方向分化的影响。方法采用不同方法诱导第三代人骨髓MSCs向成骨细胞分化,对照组:采用基础诱导培养液:地塞米松(10^(-8) mol/L)、β-甘油磷酸钠(10 mmol/L)、L-抗坏血酸(50μg/L);大黄素组:基础诱导培养+大黄素(10^(-6) mol/L);大黄素rhTGF-β1组:基础诱导培养液+大黄素(10^(-6) mol/L)+重组人转化生长因子-β1(recombinant human transforming growth factor-betal,rhTGF-β1)5 ng/ml。结果大黄素和rhTGF-β1对人骨髓MSCs增殖的影响:对照组和大黄素组与大黄素+rhTGF-β1组比较均具有显著差异(P<0.05)。大黄素和rhTGF-β1对人骨髓MSCs分化的影响:对照组、大黄素组与大黄素+rhTGF-β1组比较均具有显著差异(P<0.01)。结论基础诱导培养液加rhTGF-β1 5 ng/ml和大黄素(10^(-6) mol/L是理想的具有临床实用价值的成人骨髓MSC体外诱导为成骨细胞的培养体系。
Objective To investigate the effects of emodin on the differentiation of human bone marrow mesenchymal stem ceils （MSCs） into osteoblast in vitro. Methods Different methods were used to induce the third - passage MSCs to dif- ferentiate into osteoblast in vitro. Control group ： dexamethasone ＋ β - glycerophosphate ＋ L - ascorbic acid ; emodin group ： dexamethasone ＋ β - glycerophosphate ＋ L - ascorbic acid ＋ emodin; emodin ＋ rhTGF - β1 group： dexametbasone ＋ β - glycerophosphate ＋ L - ascorbic acid ＋ emodin ＋ TGF - β1. Results Emodin and rhTGF - β1 on the proliferation of MSCs had no compared with emodin ＋ rhTGF - β1 group, there were significant differences in Control group and emodin ＋ rhTGF- β1group, respectively（P 〈 0. 05）. Emodin on the differentiation of MSCs had no compared with emodin ＋ rh TGF -β1 group, both Control group and emodin group, had significant differences. Conclusion The basic induction medium combined the Emodin and rhTGF - β1was the more valid culture environment on the differentiation of the adult human bone marrow MSC to osteoblast in vitro.
出处
《中国医学创新》
CAS
2010年第12期5-7,共3页
Medical Innovation of China
