摘要
对黄瓜抗霜霉病品种东农129进行霜霉菌诱导,以稳定表达的18S RNA基因做对照,用RT-PCR半定量的方法分析黄瓜抗病基因同源序列(RGAs)的表达情况。结果表明,霜霉菌能诱导RGA基因的表达,经霜霉菌诱导后多数RGA基因相对表达量增强。说明了RGA基因的表达情况和霜霉菌密切相关。用半定量RT-PCR研究RGA基因的表达具有操作简单,特异性高,可靠性强的优点。
Downy mildew resistance of cucumber Dongnong129 was induced with P. cubensis by using 18S gene of stable expression as the control. The experiment used RT-PCR semi-quantitative method to analyze the expression of cucumber resistance gene analogs （RGAs）. The results showed that the P. cubensis could induce the expression of RGA gene. The relative expression of the majority of RGA gene enhanced after inducement. It illuminated that the gene expression of RGA was closely related with the P. cubensis. The method of semi-quantitative RT-PCR was simple, high specificity and reliability so as to study the RGA gene expression.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2010年第4期24-28,共5页
Journal of Northeast Agricultural University
基金
哈尔滨市科技创新人才研究专项资金(RC2006QN001028)
