摘要
目的研究原发性肝癌组织中凋亡抑制蛋白(Survivin蛋白)及β-连环素蛋白(β-catenin蛋白)的表达及其临床意义。方法应用免疫组织化学染色法对47例原发性肝癌组织、10例肝硬化组织、4例正常肝组织中Survivin及β-连环素蛋白的表达情况进行检测,并结合原发性肝癌临床生物学特性进行分析。结果在正常肝组织中Survivin蛋白无表达,β-catenin蛋白无表达;在肝硬化组织中Survivin蛋白无表达,2例(20%)β-catenin蛋白表达;在47例肝癌组织中30例(63.8%)Survivin蛋白表达阳性,31例(65.9%)β-catenin蛋白出现表达(组间比较P<0.01)。Survivin、β-catenin蛋白表达与患者的年龄、性别、肿瘤大小无关。Survivin蛋白在原发性肝癌复发组的表达率为92.9%(26/28),无复发组的表达率为21.1%(4/19);β-catenin蛋白在原发性肝癌复发组中的异常表达率为89.2%(25/28),在无复发组的表达率为31.6%(6/19)(组间比较P<0.05)。在Survivin蛋白表达阳性的30例原发性肝癌组织中有28例(93.3%)β-catenin蛋白现出异常表达,而在β-catenin蛋白出现异常表达31例原发性肝癌组织中有28例(90.3%)Survivin蛋白表达阳性,两种蛋白在原发性肝癌组织的的表达具有一致性,且呈正相关(r=0.6324)。结论Survivin及β-catenin蛋白与原发性肝细胞癌复发密切相关,它们可能成为预测肝癌术后有无复发的一项指标。Survivin与β-catenin蛋白的表达在原发性肝细胞性肝癌中具有密切的关系,且呈正相关。
Objective To study the expression and the clinical signincance of Surviving protein and β-catenin protein in primary hepatocellular carcinoma（HCC）. Methods lmmunohistochemistry was used to study the expression patterns of Survivin protein and β-catenin protein in 47 cases of HCC,10 cases of cirrhotic tissues and 4 cases of normal liver tissues. Results No expression of Survivin in the normal liver tissues or liver cirrhosis tissues,but in 30 of 47（63.8%）cases of HCC was found,while the abnormal expression of β-catenin was found in 2 of 10 cases（20%） of liver cirrhosis tissues and 31 of 47 cases（65.9%） of HCC,showing significant difference（P〈0.01） in the expression patterns of Survivin and β-catenin protein among three different tissues.The expression of Survivin and β-catenin was not correlated to age,sex and tumor size.The expression ratios of Survivin were 92.9%（26/28） in the recurrent group of HCC and 21.1%（4/19） in the non-recurrent,while the abnormal expression ratios of β-catenin were 89.2%（25/28） and 31.6%（6/19）indicating the signifcant difference（P〈0.05） in 2 protein expression between the recurrent and non-recurrent groups.28 of 30（93.3%） cases Of HCC with positive Survivin expression revealed abnormal expression of β-catenin,whereas 28 of 31（90.3%） cases of HCC with abnormal expression of β-catenin showed positive Survivin expression,suggesting the paralled expression of the two protein with a positive correlation（r=0.6324）.Conclusion The high expression of Survivin protein and the abnormal expression of β-catenin protein in HCC suggest that both protein play an important ro1e in the generation and development of HCC,and are correlated with the recurrence of HCC.A high expression of Surviving protein and an abnormal expression of β-catenin protein can be used as the molecular biological index to determine the recurrence of HCC.In HCC,expression of Survivin and abnormal expression of β-catenin are paralled with positive correlation.
出处
《南华大学学报（医学版）》
2010年第1期65-69,共5页
Journal of Nanhua University(Medical Edition)
基金
湖南省自然科学基金(08JJ3032)
