摘要
目的 着色性干皮病互补基因D（xeroderma pigmentosum group D,XPD）是一种重要的DNA损伤修复基因,其常见的多态是位于751密码子的A→C颠换和312密码子G→A转换.本研究旨在探讨XPD基因751位点和312位点单核苷酸多态性与非吸烟女性肺癌易感性的关系.方法 采用病例-对照研究方法,纳入非吸烟女性肺癌患者222人和对照222人.以聚合酶链反应-限制性片段长度多态性方法分析XPD基因Lys751Gln和Asp312Asn多态基因型.结果 携带至少1个751Gln等位基因者和携带至少1个312Asn等位基因者患肺癌的风险均显著增高,调整OR分别为3.36（95%CI为2.29-4.90）和1.83（95%CI为1.16-2.91）.结论 XPD基因Lys751Gln和Asp312Asn多态是非吸烟女性肺癌的遗传易感因素.
Objective Xeroderma pigmentosum group D （XPD）is one of the important DNA repair genes. XPD polymorphism at Lys751 Gin site and Asp312Ash site have been shown to alter XPD protein function, modulate DNA repair capacity and therefore affect cancer risk. The aim of this study is to explore the relationship between XPD polymorphism and susceptibility to lung cancer in nonsmoking female by a population - based case -control study. Methods There were 222 female patients who were diagnosed with lung cancer from Heilongjiang Tumor Hospital, and the control group included 222 healthy volunteers who were obtained from community centers. XPD genotypes of cases and controls were determined by PCR RFLP method. Unconditional logistic regression analysis was performed to calculate the odds ratios （OR）with 95 confidence intervals （CI）for estimating the association between certain genotypes and lung cancer. Results All of the subjects in this study were nonsmoking females who living in Harbin more than 30 years. The risk of lung cancer was higher in those with the Lys/Gln or Gin/Gin genotype than in those with the Lys/Iys genotype and adjusted OR was 3.36（95 CI：2.29 - 4.90） and in those with the Asn/Asn or Asp/Asn genotype than in those with the Asp/Asp genotype and adjusted OR was 1.83（95 CI： 1.16 - 2.91 ）. Conclusion The findings indicated that the Lys751Gln and Asp312Asn polymorphisms in XPD gene are associated with the risk of lung cancer in nonsmoking females.
出处
《实用肿瘤学杂志》
CAS
2010年第2期108-111,共4页
Practical Oncology Journal
基金
国家自然基金项目资助（30371244）
黑龙江省卫生厅项目资助（2005-212）
