摘要
为建立犬细小病毒(CPV)环介导等温扩增(LAMP)检测方法,实现CPV的早期快速诊断,本研究根据GenBank登录的CPV VP2基因序列,在其序列保守区域设计LAMP引物,利用CPV基因组DNA为模板进行扩增。结果表明:LAMP方法检测灵敏度达到10-1TCID50/mL;并且与其它细小病毒等无特异性扩增,表现出良好的特异性。与PCR技术相比,LAMP法操作更加简单方便,更适合基层和实验室的快速检测。
A loop-mediated isothermal amplification assay（LAMP） for rapid and accurate detection of canine parvovirus（CPV） was developed using specific primers derived from VP2 gene of CPV. The assay was specific for CPV and did not amplify any PCR product from porcine parvovirus,gosling plague virus,canine distemper virus. It was highly sensitive and had a detection limit of 2 to 3 DNA copies. The LAMP assay was simple and more convenient than PCR method,which could provide a useful technique for CPV clinic sample detection.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2010年第4期263-266,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
国家"863"计划(2008AA10Z411)
中央级公益性科研院所基本业务费专项(0032007008)
北京市科委基金项目(Z07010501780701)
