摘要
通过PCR技术分别在已有的几丁质酶基因和内切葡聚糖酶基因前端加入了SD序列,通过酶切、连接将上述两个基因串连,构建了自杀性转座重组质粒,为进一步构建转双价基因生防菌奠定了基础。
Chitianse and endoglucanase are two factors with synergistic effects involved in antifungus system. It is the key and common technique for the engined biocontrol bacteria integrated with the suicide recombinant plasmid constructed by the two genes. Based on the sequence of chi113 and egl25, SD seuquences were added to the front of the two genes through PCR amplification. The suicide transposable plasmid was constructed through connection in series by enzyme-digestion.
出处
《湖北农业科学》
北大核心
2010年第4期775-778,共4页
Hubei Agricultural Sciences
基金
山东省自然基金项目(Y2006D18)
中国援助巴西项目(2009DFA32340)
国家"863"计划现代农业技术领域重大项目(2006AA10A211)
关键词
伯克氏菌
双价基因
自杀性质粒
串连
Burkholderia
double genes
suicide plasmid
connection in series
