摘要
目的正交实验法优选福寿螺多糖的最佳提取工艺。方法以多糖含量为指标,对提取过程中的醇沉浓度、溶液pH值、浸提温度及浸提时间4个因素进行优选研究。结果最佳提取条件为:加无水酒精至醇沉浓度为75%、溶液的pH=9.0、浸提温度50℃、浸提时间8 h。结论福寿螺多糖碱浸醇沉工艺经济,有效,可行。
Aim To choose the optimum extraction process of polysaccharide from Pila gigas Spix.Methods The content of polysaccharide was used to evaluate the factor levels.Four technological factors of alkali extraction process,including alcohol concentration,extraction temperature,pH value of solution and extraction times were optimized by orthogonal test.Results The ideal extraction process were as follows：dehydrated alcohol was added to the fluid until the alcohol content up to 75%,with a solution pH value of 9.0,extraction temperature at 50 ℃,and an extraction time of 8 h.Conclusion The optimum extracting procedure was economical,effective,and feasible.
出处
《安徽医药》
CAS
2010年第5期529-530,共2页
Anhui Medical and Pharmaceutical Journal
关键词
福寿螺
多糖
提取
正交实验
pila gigas spix
polysaccharide
extraction
orthogonal test
