摘要
目的:探讨被动吸烟对雌性Wistar大鼠卵巢结构和功能的影响。方法:雌性Wistar鼠32只,随机分为对照组和被动吸烟组,对照组在自然环境下饲养,吸烟组在自制的吸烟箱内被动吸烟3个月,吸烟三个月后,对两组分别进行秤重,然后处死。用测量仪器测量母鼠卵巢的大小、重量,并做光、电镜观察,免疫组织化学检测ER、PR、FSHR、LHR在卵巢中的表达,所得数据进行统计学处理。结果:吸烟三个月大鼠卵巢充血性囊性退变,皮质萎缩,卵泡颗粒细胞排列紊乱;生长卵泡的颗粒细胞松散变性,排列不整齐;卵泡数减少,成熟的卵母细胞数目明显减少;髓质血管收缩,减少;间质疏松。②电镜对照组可见多个卵泡细胞,细胞内有空白颗粒及高密度颗粒。吸烟组可见多个颗粒细胞层,内有增粗的内质网、核糖体;核周隙局部增宽,线粒体模糊;线粒体水样变、空泡样变,线粒体嵴断裂、模糊;内质网脱颗粒样变。与没有吸烟的正常大鼠B组比较,吸烟大鼠的ER、PR、FSHR、LHR减少(P<0.05)。结论:被动吸烟组雌性卵巢充血性囊性退变,皮质萎缩,卵泡颗粒细胞排列紊乱。
Objective：Effects of passive smoking on ovarian function in female wistarrats and its impact on embryonic development. Methods：32 female Wistar rats were randomly divided into control group and the passive smoking group and the control group reared in the natural environment, smoking in the home-made smoking group box 3 months of passive smoking, smoking after three months of weighing the two groups separately, and then executed. Rats by measuring instruments measuring ovarian size, weight, and so light, electron microscopy, immunohistochemical detection of ER, PR, FSHR, LHR expression in the ovary, from statistical data. Results：The smoking three months in rats with congestive ovarian cystic degeneration, cortical atrophy, follicular granulosa cells disorder; the growth of follicle degeneration of the granulosa cells loosely arranged irregular; reduce the number of follicles and mature oocytes significantly reduced the number of ; medullary vasoconstriction, reduced; interstitial osteoporosis. ② electron microscopy shows that the control group a number of follicle cells, granule cells are blank and high-density particles. Group can be seen smoking a number of granulosa cell layer, with thickening of the endoplasmic reticulum, ribosomes; partial gap widened perinuclear.mitochondria blurred; mitochondrial water change, vacuolization degeneration, mitochondrial cristae fragmentation, fuzzy; in endoplasmic reticulum degranulation degeneration. And no smoking group B in normal rats, smoking rat ER, PR, FSHR, LHR reduced （P〈0.05）. Conclusion：Effects of passive smoking on ovarian function of female influence.
出处
《现代生物医学进展》
CAS
2010年第5期865-868,F0003,共5页
Progress in Modern Biomedicine
基金
山东省医药卫生科技发展计划项目(2007HZ021)
