摘要
目的:建立大血藤的最佳提取方法。方法:利用HPLC法,比较超声提取、回流提取、不同溶媒、不同提取时间对绿原酸提取率的影响。结果:50%甲醇,回流提取1.5h为最佳条件。结论:该方法稳定、重现性好,可作为大血藤的最佳提取工艺。
Objective:To study the optimum extraction in Sargentodoxa cuneata.Methods:The effections of Chlorogenic Acid's extraction rate are compared with ultrasonic extraction,refluxing extraction,different rnenstru-um and different time by HPLC.Result :he optimum extraction are added 50% methanol,extracted 1.5 h by refluxing extraction.Conclusion:The method was stabile ,good reproducibility, and can be used of the optimum extraction of Sargentodoxa cuneata.
出处
《中医临床研究》
2010年第7期75-76,共2页
Clinical Journal Of Chinese Medicine
关键词
大血藤
HPLC
绿原酸
回流提取法
Sargentodoxa cuneata
HPLC
Chlorogenic Acid
refluxing extraction