摘要
目的从雌激素受体α(ERα)的角度探讨自然发情小鼠与诱导发情小鼠的子宫内膜上,雌激素受体α表达是否受内源雌激素的特异诱导而变化,两者之间是否存在差异。方法27只同日龄母鼠,根据处理方式的不同随机分为3个组:自然发情假孕组(对照组)、诱导发情处理假孕组和自然发情假孕第1天摘除卵巢组,3个组的小鼠在见栓后第4、6、8天分别取样后,采用免疫组织化学法观察小鼠子宫内膜中雌激素受体α的表达情况。结果免疫组化结果显示,3个处理组的小鼠子宫内膜上皮细胞核、胞质都有ERα存在,且主要表达在腺上皮;见栓第4、6、8天时,诱导发情处理组小鼠子宫内膜的ERα阳性率均显著高于自然发情组和自然发情第1天摘除卵巢组(P<0.05);见栓第8天时,自然发情处理组小鼠子宫内膜中的ERα阳性率与自然发情第1天摘除卵巢组差异不显著(P>0.05),但见栓第4、6天时两者阳性率差异显著,自然发情处理组小鼠子宫内膜中的ERα阳性率显著高于自然发情第1天摘除卵巢组(P<0.05)。结论诱导发情处理的小鼠子宫内膜,其表面雌激素受体α表达显著高于自然发情小鼠,且两者都受其内源性雌激素的特异诱导而变化。
Objective To investigate the distribution of estrogen receptor α（ERα）in the endometrium between the mice treated by estrus synchronization and in natural estrus,and analyze whether such kind of ERα distribution is solely induced by endogeneous estrogen.Methods According to the different treatments,total 27 ICR mice were divided into three groups：（1） the group in natural estrus and pseudocyesis（the control group）,（2 ） the group treated by estrus synchronization and pseudocyesis,（3） the group in natural estrus,pseudocyesis and being ovariectomized at the first day of estrus.Results The results of immunohistochemistry showed that the estrogen receptor α distributed in both nucleus and cytoplasm,and ERα mainly expressed in glandular epithelium（GE）.On day 4,6,8,the positive staining cell number in the estrus synchronization group was much higher than those of natural estrus（P 0.05）.On day 8,the positive staining cell number in the group of natural estrus and pseudocyesis and being ovariectomized at the first day of estrus,was not significantly different（P 0.05）,but on Day 4 and 6,these data of the natural estrus group were significantly higher than those of the pseudocyesis one being ovariectomized at the first day of estrus（P 0.05）.Conclusion The expression of ERα in mouse uterine endometrium by estrus synchronization is much higher than that of the natural estrus one.The ERα distribution is mainly induced by their endogeneous estrogen.
出处
《中国实验动物学报》
CAS
CSCD
2010年第2期168-171,F0003,共5页
Acta Laboratorium Animalis Scientia Sinica
基金
北京市自然基金重点项目"胚胎滋养层干细胞的功能性分析"(项目批准号:5091002)
国家"十一五""863计划"重点项目"家畜卵泡高效诱导发育技术研究与应用"(项目批准号:2008AA101003)
国家"十一五科技支撑计划"项目"肉羊高效育种关键技术的研究与应用"(项目批准号:2008BADB2B04-4)
