摘要
目的通过改良接种方式建立兔肝癌模型,分析并观察该肿瘤的生长特性。方法经肝包膜植入瘤组织块(约106～108个瘤细胞)接种于20只兔的肝左叶,建立兔肝癌模型。观察:①肿瘤在实验兔中生长7、10、14、17和21d时的体积(B超测),并计算肿瘤生长率。②大体及光镜下观察瘤组织形态特征。结果瘤体呈指数性生长,在接种17d后生长迅速。组织病理表明该瘤在肝组织中浸润式生长,肿瘤体积增长快于直径增长,其性状与VX2鳞状细胞癌特征相似。结论瘤块种植是建立兔肝癌模型的首选方式,该模型是肝癌的基础及临床的理想动物模型。
Objective To establish a rabbit VX2 liver tumor model by transplanting tumor tissue mass into the rabbit’s liver,to analyze and observe the growing features of the liver tumor.Methods The tumor tissue mass（about 10^6-10^8 VX2 liver tumor cells）was inoculated into the left hepatic lobe in 20 rabbits to establish rabbit VX-2 hepatic carcinoma model.The observation included the following two respects. （1）The tumor’s volume at 7,10,14,17 and 21 days after the procedure was measured by ultrasonography and the growth rate of tumor was calculated.（2）The morphological feature of the tumor was inspected both macroscopically and microscopically.Results The growing pattern of the tumor was compatible with the exponential curve.Seventeen days after transplantation the increase rate of the tumor volume was much higher than that of the tumor diameter.Histopathologically,the growing pattern of the tumor took the form of infiltrative way,with its appearance being quite similar to the VX2 squamous cell carcinoma.Conclusion Transplantation of tumor tissue mass is the technique of first choice to establish the VX2 liver carcinoma model in rabbits.This experimental model is a very ideal animal form for both clinical and fundamental studies of liver carcinoma.
出处
《介入放射学杂志》
CSCD
北大核心
2010年第3期211-213,共3页
Journal of Interventional Radiology
关键词
兔VX2
肝癌
移植方式
生长特性
rabbit VX2 transplanted tumor model
hepatic carcinoma
transplanting method
growing feature
