摘要
化妆品以d8-NDELA为内标,经提取净化后,采用HPLC-MS/MS测定NDELA(N-亚硝基二乙醇胺)。提取净化时水溶性样品以水作溶剂,用C18柱固相萃取,水不溶性样品用二氯甲烷进行液液萃取。该方法线性范围为5.0 ng/mL～80 ng/mL,NDELA定量限为50μg/kg,回收率85%～104%。实验结果表明,该方法灵敏、准确、重复性好。
The determination of the nitrosamine NDELA in cosmetic samples is carried out by liquid chromatography and mass spectrometry after extraction and clean-up in the presence of d8-NDELA used as internal standard. Clean-up is performed either using solid phase extraction （SPE clean-up） with a C18 cartridge or liquid-liquid extraction using dichloromethane （DCM clean- up） when the samples are not dispersible in water. Linearity of method is 5.0 ng/mL-80 ng/mL, the limit is 50 μg/kg and the recovery is 85%-104%. The method appeared to be accurate and reproducible.
出处
《香料香精化妆品》
CAS
2010年第2期28-29,32,共3页
Flavour Fragrance Cosmetics
