摘要
目的观察病毒性脑膜脑炎、化脓性脑膜脑炎患者急性期和恢复期脑脊液和血清中的IFN-γ、IL-10和S-100B蛋白含量变化并探讨其意义。方法32例病毒性脑膜脑炎患者(病脑组)和28例化脓性脑膜脑炎患者(化脑组)作为观察组,30例与观察组相匹配的紧张性头痛或神经症患者及健康体检者作为对照组,采用双抗体夹心ELISA法分别检测其脑脊液和血清中IFN-γ、IL-10和S-100B蛋白的浓度。结果病脑组和化脑组患者急性期脑脊液和血清IFN-γ、IL-10、S-100B蛋白水平显著高于恢复期和对照组(P<0.01),急性期病脑组患者脑脊液和血清IFN-γ水平显著高于化脑组(P<0.01),急性期化脑组患者脑脊液和血清IL-10、S-100B蛋白水平显著高于病脑组(P<0.01);急性期脑脊液和血清IFN-γ/IL-10比值关系为:病脑组>对照组>化脑组(P<0.01);急性期昏迷患者血清和脑脊液中S-100B蛋白浓度显著高于无昏迷患者(P<0.01)。结论IFN-γ、IL-10、S-100B蛋白参与了中枢神经系统感染性疾病的病理过程,脑脊液或血清中的S-100B蛋白浓度反映了神经胶质细胞的损害程度,脑脊液和血清中IFN-γ、IL-10及IFN-γ/IL-10比值的测定有助于病毒性脑炎与化脓性脑膜炎的鉴别诊断。
Objective To observe changes of IFN-γ,IL-10 and S-100B protein level in cerebrospinal fluid and serum in patients with viral meningoencephalitis and suppurative meningoencephalitis in the acute and recovery phase,and to explore its significance.Methods 32 cases with viral meningoencephalitis（VMgroup） and 28 cases with purulent meningoencephalitis（PM group） were collected as the observed group,and 30 cases with tension headache or neurological disorders or health examinaters were collected as the control group.IFN-γ,IL-10 and S-100B protein concentration in cerebrospinal fluid and serum were detected by Double-antibody sandwich ELISA.Statistical analysis was performed by SPSS11.5 statistical package.Results The acute phase had significantly higher concentration of IFN-γ,IL-10 and S-100B protein in cerebrospinal fluid and serum than the recovery phase and control group had（P0.01）.VM group had significantly higher concentration of IFN-γ in cerebrospinal fluid and serum than PM group had in the acute phase（P0.01）.PM group had significantly higher concentration of IL-10 and S-100B protein in cerebrospinal fluid and serum than VM group had in the acute phase（P0.01）.Ratio of IFN-γ/IL-10 ： VM the control group PM（P0.01）.The coma patients in the acute phase had significantly higher concentration of S-100B protein than no coma patients had in the acute phase（P0.01）.Conclusion IFNγ,IL-10 and S-100B protein involve in the pathological process of the central nervous system infectious diseases.Concentration of S-100B protein in cerebrospinal fluid or serum reflects the extent of damage of glial cells.Detection of IFN-γ、IL-10 in cerebrospinal fluid or serum and the ratio of IFN-γ/IL-10 can help identify viral encephalitis and purulent meningitis diagnosis.
出处
《宁夏医科大学学报》
2010年第2期207-210,共4页
Journal of Ningxia Medical University
关键词
颅内感染
后脊液
白细胞介素-10
Γ-干扰素
S-100B蛋白
intracranial infections viral encephalitis purulent meningitis interleukin-10 γ-interferon S-100B protein
