摘要
外担子菌属（Ｅｘｏｂａｓｉｄｉｕｍ）的真菌引起树木的细胞增生性和肥大性病害。本研究基于ＰＳＡ培养基上菌落的形态和性状的模糊解析，以及分离菌株在不同温度、ｐＨ值、氮源、碳源下生长曲线的模糊解析和２８ＳｒＤＮＡＰＣＲＲＦＬＰ解析，分布于日本的１２个种和１个变种的外担子菌属真菌的３５个分离菌株被分类为１８个菌群。其中９个菌群和传统分类的种是一致的，这些种包括：坏损外担子菌（Ｅ．ｖｅｘａｎｓＭａｓ）、网状外担子菌（Ｅ．ｒｅｔｉｃｕｌａｔｕｍＩｔｏｅｔＳａｗａｄａ）、山茶外担子菌（Ｅ．ｃａｍｅｌｉａｅＳｈｉｒａｉ）、Ｅ．ｃｙｌｉｎｄｒｏｓｐｏｒｉｕｍＥｚｕｋａ、Ｅ．ｓｈｉｒａｉａｎｕｍＰ．Ｈｅｅｎ．、Ｅ．ｈａｃｈｉｊｏｅｎｓｅＯｔａｎｉ，ＫａｋｉｓｈｉｍａｅｔＩｉｊｉｍａ、Ｅ．ｂｉｓｐｏｒｕｍＳａｗａｄａｅｔＥｚｕｋａ、Ｅ．ｐｉｅｖｉｄｉｓｏｖａｌｉｆｏｌｉａｅＳａｗａｄａ和Ｅ．ｓｙｍｐｌｏｃｉｊａｐｏｎｉｃａｅＫｕｓａｎｏｅｔＴｏｋｕｂｕｃｈｉ；细丽外担子菌（Ｅ．ｇｒａｃｉｌｅ（Ｓｈｉｒａｉ）Ｓｙｄｏｗ）的７个菌株分化为４个菌群，表现了种内的地理、寄主分化；日本外担子菌（Ｅ．ｊａｐｏｎｉｃｕｍＳｈｉｒａｉ）?
The fungus belonging to the genus Exobasidium (Basidiomycotina, Ustomycetes, Exobasidiales) is known as the pathogen of cell's hyperplastic and hypertrophic disease of several tree plants. In this study, thirty five isolates (isolated from Japan) of twelve species and one variety of the genus were divided into eighteen groups based on the fuzzy analysis of cultural properties (shapes and characters of colonies; growth curve of the isolates under different temperatures, pHs, nitrogen sources and carbon sources) and the analysis of 28S rDNA PCR RFLP. Among the eighteen groups, nine groups were identical with the species identified by the traditional taxonomy. These species are E.vexans Mass、E.reticulatum Ito et Sawada E.camelliae Shirai E.cylindrosporium Ezuka E.shiraianum P.Heen. E.hachijoense Otani, Kakishima et Iijima E.bisporum Sawada et Ezuka E.pievidis ovalifoliae Sawada and E.symploci japonicae Kusano et Tokubuchi. Tested seven isolates of E.gracile (Shirai) Sydow were differentiated into four groups that showed the differentiation related to geographical environment and hosts in the species. Tested seven isolates of E.japonicum Shirai were differentiated into five groups that showed the differentiation related to hosts in the species. Tested isolates of E.yoshinagai P.Heen., E.otanianum Ezuka var. satsumense Zhang et Arai and the three isolates of tested seven isolates of E.japonicum were classified into one group.
出处
《林业科学》
EI
CAS
CSCD
北大核心
1998年第4期59-71,T001,共14页
Scientia Silvae Sinicae
关键词
外担子菌
培养性质
RFLP
模糊解析
分类学
Exobasidium, Cultural properties, RFLP, Fuzzy analysis, Taxonomy Plate I. Electrophoretic photographs showing the result of 28S rDNA PCR RFLP of tested isolates. 1.Electrophoretic pattern of PCR amplifiede 28S rDNA of tested isolates. 2.Electropho
