摘要
目的:探讨乌司他丁对急性坏死性胰腺炎(ANP)大鼠血清巨噬细胞游走抑制因子(MIF)水平的影响。方法:健康Wister大鼠52只分为3组:正常对照组(C组,12只)、ANP组(A组,20只)和乌司他丁治疗组(U组,20只)。A组大鼠经十二指肠乳头逆行注射3.5%牛黄胆酸钠诱导构建ANP模型;U组同法诱导构建ANP模型后,经门静脉给予乌司他丁40000U/kg;C组开腹仅翻动胰腺和十二指肠。各组于术后3、6、12和24h分批处死大鼠,切取胰腺组织,采集下腔静脉血。对各组不同时点大鼠胰腺组织进行胰腺组织病理学评分,并测定血清淀粉酶和血清MIF水平。结果:A组各时点胰腺组织病理学评分及血清淀粉酶、血清MIF水平明显高于C组(P<0.01);U组各时点胰腺组织病理学评分、血清淀粉酶和血清MIF水平均低于A组(P<0.05或P<0.01),A组血清MIF水平与胰腺组织病理学评分成正相关(r=0.513,P<0.05)。结论:MIF可能在ANP的发病中发挥重要作用;乌司他丁下调MIF水平,从而减轻胰腺病理损伤,改善重症急性胰腺炎预后。
Objective： To explore the effects of ulinastatin on the level of MIF in rats with acute necrotic pancreatitis. Method： 52 healthy Wister rats were randomly divided into three groups： normal control group（group C, 12）, ANP group（group A, 20）and UTI group（group U, 20）. Severe acute pancreatitis rat model in group A were induced by injection of 315 % sodium taurocholate through retrogradely common biliopancreatic ducts via papilla duodeni. After inducing the rat model of ANP through the way above, rats in group U were treated by ulinastatin through portal vein injection. Pancreas and duodenum were only flipped after opening abdominal cavity in group C. Then rats were killed at 3rd, 6th ,12th ,24th hour after operation respectively. Cut the belly open at once, and draw blood in postcava. The levels of serum MIF were determined with ELISA. Blood amylase was detected through biochemistry instrument. Resected pancreas tissues was scored according to the standard of Kusske. Result： Compared to the normal control group, the level of serum MIF , blood amylase and histopathological scores were significantly increased in ANP group, P0.01. Additionally, it was significantly down-regulated in group U, P0.01 or P0.05. Positive correlation was found between histopathological scores and serum levels of MIF, P0.05. Conclusion： The level of serum MIF might play an important part in experimental ANP. Ulinastatin might improve the prognosis of ANP by inhibiting enzymatic activity and down-regulated the level of MIF.
出处
《中国现代普通外科进展》
CAS
2010年第1期12-15,共4页
Chinese Journal of Current Advances in General Surgery
基金
山东省教育厅科技研究计划项目(J06L51)
滨州医学院科技计划项目(BY2005KJ12)
