摘要
目的探讨RASSF1A基因的表达对肝细胞肝癌化疗药物敏感性的影响。方法利用已构建成功的稳定表达野生型和突变型RASSF1A基因的肝癌细胞株QGY-7703,化疗药物Mitomycin、Adriamycin、Etoposide、5-Fluorouracilur、Cisplatin分别作用各细胞株后,比较生长抑制率、细胞周期及P53、P21、Bax、Caspase-3蛋白的表达水平。结果野生型RASSF1A的表达可提高Mitomycin诱导的肝癌细胞生长抑制率、凋亡发生率(P<0.05)和Caspase-3的活性,对P53、P21、Bax基因的蛋白表达水平没有影响。结论野生型RASSF1A基因可提高肝癌细胞对Mitomycin的敏感性。
Objective To explore the effect of RASSF1A on the chemosensitivity of human hepatocellular carcinoma （HCC） cell line QGY-7703.Methods The QGY-7703 cells expressing RASSF1A gene （wild type or mutant） stably were used in this study. The cells were treated with the antitumor drugs including Mitomycin, Adriamycin, Etoposide, 5-Fluorouracilur and Cisplatin. Then the rate of cell growth inhibition, changes of cell cycle and expression levels of p53, p21, bax and caspase-3 were measured.Results Expression of wild-type RASSF1A in the QGY-7703 cells could enhance the rate of cell growth inhibition, the percentage of apoptotic cells and caspase-3 activity compared with the cells that express mutant RASSF1A after those cells were treated with Mitomycin（P〈0.05）. No significant differences of expression levels of the Bax, p53 and p21 protein were observed among those cells.Conclusion Wild-type RASSF1A expression could increase chemosensitivity of QGY-7703 cells to Mitomycin.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2010年第4期414-416,共3页
Cancer Research on Prevention and Treatment
基金
南通市社会发展科技计划资助项目(S2007044)
南通大学自然科学资助项目(07Z093)
