摘要
本研究从基因水平了解HLA-Cw*位点在中国北方汉族人群中的分布频率,分析HLA-Cw*等位基因的群体遗传学特点及基因频率分布的地区差异。采用PCR-SSP分型技术对420名中国北方汉族人群HLA-Cw*位点进行高分辨基因分型,并对其分布规律进行统计学分析。结果表明:共检出30个HLA-Cw*等位基因,分布频率最高的有Cw*0102;0702;0602(0.1776;0.1217;0.1150),其他频率较高的等位基因依次为Cw*0304,0801,0303,0302,0401,1402。首次在该人群中检测到HLA-Cw*0506,0810,1510,1601和1701较少见等位基因。统计分析表明,HLA-Cw位点基因型分布符合Hardy-Weinberg平衡(p>0.05)。结论:采用高分辨等位基因分型,可准确了解HLA-Cw*位点等位基因在我国北方汉族群体中的分布规律和特征,为HLA-Cw*座位的相关研究提供了依据。
This study was purposed to investigate the frequencies of HLA-Cw ＊ loci in China Northern Han population at gene level and to analyze the population genetic characteristics of HLA-Cw ＊ alleles and distribution difference of gene frequency in regions. The high resolution genotyping for HLA-Cw ＊ loci of 420 cases in China Northern Han population was performed by using PCR-SSP typing technique and their distribution regularity was analyzed statistically. The results showed that 30 HLA-Cw＊ alleles were detected, among which the frequency of Cw＊ 0102 （0. 1776）, 0702（0. 1217）, 0602 （0. 1150） were highest; other alleles with higher frequency were as follow in proper order： Cw ＊ 0304, 0801, 0303, 0302, 0401, 1402. The rare observed HLA- Cw＊ 0506,0810,1510,1601 and 1701 were detected firstly in this population. The statistical analysis indicated that the genotype distribution of HLA-Cw ＊ loci coincides with the Hardy-Weinberg test. In conclusion, application of high resolution allele typing can accurately understand the distribution regularity and characteristics of HLA-Cw ＊ alleles in China Northern Han population which provides the basis for research related with HLA-Cw^＊ loci.
出处
《中国实验血液学杂志》
CAS
CSCD
2010年第2期486-489,共4页
Journal of Experimental Hematology
基金
天津市科技创新专项资金,编号08FDZDSH03000
