摘要
将52名0～3岁佝偻病患儿和58名健康几分成5组,测定其血清总碱性磷酸酶(t-ALP)活性,并对血清ALP同工酶进行分析.佝偻病患儿血清t-ALP随着年龄增长而逐渐降低,t-ALP患儿组明显高于健儿组(P<0.01);56℃15min热灭活剩余酶活性,患儿组明显低于健儿组(P<0.01),也低于骨型ALP.增高的诊断指标≤12.7%.0.01mol/LL-本丙氨酸对血清ALP的抑制,患儿组和健儿组无显著性差异(P>0.05);3mol/L脲对血清ALP的抑制影响,0～1.5岁三个年龄组存在显著性差异(P<0.05),而1.5～3岁二个年龄患儿组与健儿组无显著性差异(P>0.05);根据电泳迁移率Rf值,得出主带是骨型ALP.凝胶光密度扫描结果认为主带面积,患儿与健康儿无差别(P>0.05).佝偻病患儿血清t-ALP增高是由骨型ALP引起的.
Fifty two children with rickets and 58 healthy children, all under 3 years of age, were divided into 5 groups for study. The results showed that total alkaline phosphatases (t-ALP) in the rickets were significantly higher than in the controls (P<0.01) . The remaining activities of heating 56℃ for 15 minutes in the rickets serum were not only significantly lower than the control (P<0.01), but also lower than the diagnostic limits (12.7%) of bone alkaline phophatase. There were no significant differences in serum inhibited by 0.01mol/L l-phenylalamide (P>0.05). But when sera were inhibited by 3mol/L urea, there were significant differences in the first three age groups(P<0.05), but not in the last two groups (P>0.05). Electrophoretic flowing rate values(Rf) showed that the main peak was bone alkaline phosphatase. There were no differences between the rickets and the controls quantitated by peak area measurements from spectrophotometric scans (P>0.05). It is
concluded that the high t-ALP levels in serum of the rickets was from bone alkaline phosphatase.
出处
《青岛医学院学报》
1990年第2期100-10,共1页
Acta Academiae Medicinae Qingdao Universitatis
关键词
佝偻病
儿童
碱性磷酸酶
同功酶
Rickets
Alkaline phosphatase
Isoenzymes
Children
