摘要
目的:探讨中药方剂小青龙汤对小鼠哮喘模型气道炎症及细胞因子的影响。方法:40只BALB/c小鼠随机分为正常对照组(A组)、哮喘模型组(B组)、小青龙汤低剂量组(C组)和小青龙汤高剂量组(D组)。B、C、D组采用卵蛋白(OVA)腹腔注射致敏与雾化吸入激发制作哮喘模型。于OVA激发结束后24h收集支气管肺泡灌洗液(BALF)计数炎性细胞总数及嗜酸性粒细胞(EOS)数目,并测定BALF上清液中白细胞介素-4(IL-4)和干扰素-γ(IFN-γ)水平变化。结果:小青龙汤的干预治疗能显著降低小鼠BALF中炎性细胞总数及EOS数量;BALF上清液中IFN-γ水平明显升高,IL-4水平显著下降。D、C组与A组、B组有显著性差异(P<0.05),C组与D组结果亦存在显著性差异(P<0.05)。结论:小青龙汤能明显降低哮喘小鼠BALF中炎性细胞数量,影响细胞因子水平变化,从而改善哮喘气道炎症。
Objective： To explore the effect of Xiaoqinglong decoction on the airway inflammation and the cytokine production in asthmatic mice. Methods： Forty BALB/c mice were divided into four groups including a control group （ group A）, an asthmatic model group （group B）, low dose of Xiaoqinglong decoction group （ group C） and high dose of Xiaoqinglong decoction group （group D）. Asthma mouse models in group B, group C and group D were prepared by injecting ovalbumin （ OVA ） into the abdominal cavity and inhalation of aerosol . The number of EOS and the concent ration of IL-4 and IFN-γ in the mouse bronchoalveolar lavage fluids （BALF） were detected. Results： Xiaoqinglong decoction could significantly reduce the mumber of EOS and the concent ration of IL-4, and increase t he level of IFN-γ in the BALF of asthmatic mice. Significant difference was found between group D or C and group A or B （P〈0.05）. Group C and D also show significant difference （P〈0.05）. Conclusion： Xiaoqinglong decoction can remarkably reduce the mumber of inflammation cells in the BALF , and influence the level of cytokines so as to improve airway inflammation in the patients with asthma.
出处
《现代生物医学进展》
CAS
2010年第4期655-657,共3页
Progress in Modern Biomedicine
关键词
小青龙汤
哮喘模型
气道炎症
细胞因子
Xiaoqinglong decoction
Asthmatic model
Airwayinflammation
Cytokines
