摘要
采用球根秋海棠（ＢｅｇｏｎｉａｔｕｂｅｒｈｙｂｒｉｅｄａＶｏｓａ）的幼茎、花瓣和花梗作为外植体，以Ｍｓ为基本培养基，并附加细胞分裂素ＢＡ或激动素ＫＴ以及生长素ＮＡＡ或２，４－Ｄ．通过１个月的培养，获得愈伤组织．芽的诱导在附加ＢＡ２ｍｇ／１＋ＭＡＡ０．５ｍｇ／１＋间苯三酚１ｍｇ／１的Ｍｓ培养基上获得最高分化率．增殖阶段以附加ＢＡ０．５ｍｇ／１＋ＮＡＡ０．１ｍｇ／１的马铃薯培养基最经济有效．通过不加激素的Ｍｓ液体培养基的浅层培养得到健壮的小苗．无根小苗在１／２Ｍｓ＋ＮＡＡ０．１ｍｇ／１或不加ＮＡＡ的培养基中诱导生根．经过适当练苗，移栽的小苗即可正常生长开花．
Begonia tuberhybrieda is a famous potted flower with lower coefficient of clonal propagation and easier degeneration by seed propagation. To breed rapidly and keep original property, the authors cultured shoots, petal and pedicel explants of Begonia tuberhybrieda on Ms medium supplemented with BA( 0.5, 2 mg/1)+NAA(0.1, 0.5 mg/1) or 2, 4～D1 mg/1 separately to induce callus. The Ms medium with BA 2 mg/1+NAA 0.5 mg/1+Phloroluoin 1 mg/1 is better for differ-entiation of adventitious buds and the Potato medium with BA 0.5 mg/1+NAA 0.1 mg/1 is suitable for proliferation. The Ms liquid medium without promoting substance is for strengthening. Plantlets and the 1/2 Ms medium with NAA 1 mg/1 (or without NAA) is for inducing roots.
出处
《西南林学院学报》
1998年第2期74-78,共5页
Journal of Southwest Forestry College
关键词
球根秋海棠
组织培养
增殖
培养基
快速繁殖
Begonia tuberhybrieda
Tissue culture
Proliferation
Strengthening Plantlets
