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幼年感染细菌内毒素对成年实验性自身免疫性脑脊髓炎大鼠行为和病理的影响

Effects of Neonatal Endotoxin Exposure on Behavior and Pathology of Experimental Autoimmune Encephalomyelitis in Adult Sprague-Dawley Rats
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摘要 目的:探讨大鼠幼年时期感染细菌内毒素对其成年后实验性自身免疫性脑脊髓炎(EAE)神经功能损伤和中枢神经系统病理学的影响。方法:取同窝SD幼鼠,于出生后第3和第5天2次给予腹腔注射0.01 mg·mL^(-1)脂多糖(LPS)0.05mL或同等剂量无菌生理盐水。成年后(出生后第8周)用豚鼠脊髓匀浆免疫SD大鼠制作EAE模型,观察并比较这两组大鼠行为学与组织病理学变化。结果:与生理盐水对照组相比,LPS处理组SD大鼠EAE发病率明显降低(P<0.01),且中枢神经系统内炎症细胞浸润及神经髓鞘脱失也明显减轻(P<0.01)。结论:幼年时期感染细菌内毒素对成年SD大鼠EAE有明显的保护作用。 Aim: To investigate the effects of neonatal endotoxin exposure on behavior and pathology of experimental autoimmune encephalomyelitis in adult rats. Methods: Neonatal endotoxin exposure involved injecting Sprague-Dawley(SD) rat pups intraperitoneally with either lipopolysaccharide in 0.05ml sterile saline or an equivalent volume of sterile saline vehicle on days 3 and 5 postpartum. EAE was induced by immunizing SD rats with guinea pig spinal cord homogenate in adulthood(8 weeks of age). After immunization, the ethologic and histopathological changes between these two groups were compared. Results: In the LPS group, the incidence was obviously reduced(P〈0.01), inflammatory cell infiltration and demyelination in central nerve system were significantly decreased(P〈0.01) as compared with the saline group. Conclusion: Neonatal endotoxin exposure had protective effect on EAE in adult SD rats.
出处 《中国临床神经科学》 2010年第2期161-166,共6页 Chinese Journal of Clinical Neurosciences
基金 温州医学院重大课题(编号:XNK07001)
关键词 内毒素 脂多糖 实验性自身免疫性脑脊髓炎 动物模型 大鼠 endotoxin lipopolysaccharide experimental autoimmune encephalomyelitis animalmodel rat
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  • 1Van Amersfoort ES,Van Berkel TJ,Kuiper L Receptors,mediators,and mechanisms involved in bacterial sepsis and septic shock[J].Clin Microbiol Rev,2003,16:379-414. 被引量:1
  • 2Shanks N,Windle RJ,Perks PA,et al.Early-life exposure to en-dotoxin alters hypothalamic-pituitary -adrenal function and pre-disposition to inflammation[J].Proc Natl Acad Sci U S A,2000,97:5645-5650. 被引量:1
  • 3Walker FR,Brogan A,Smith R,et al.A profile of the immediate endocrine,metabolic and behavioural responses following a dual exposure to endotoxin in early life[J].Physiol Behav,2004,83:495-504. 被引量:1
  • 4Ellestad KK,Tsutsui S,Noorbakhsh F,et al.Early life exposure to lipopolysaccharide suppresses experimental autoimmune encepha-lomyelitis by promoting tolerogenic dendritic cells and regulatory T cells[J].J Immunol,2009,183:298-309. 被引量:1
  • 5Breivik T,Stephan M,Brabant GE,et al.Postnatal lipopolysac-charide-induced illness predisposes to periodontal disease in adulthood[J].Brain Behav Immun,2002,16:421-438. 被引量:1
  • 6Jaworek J,Leja-Szpak A,Nawrot-Porabka K,et al.Effect of neo-natal endotoxemia on the pancreas of adult rats[J].J Physiol Pharmacol,2008,59(Suppl 4):87-102. 被引量:1
  • 7Bilbo SD,Yirmiya R,Amat J,et al.Bacterial infection early in life protects against stressor-induced depressive-like symptoms in adult rats[J].Psychoneuroendocrinology,2008,33:261-269. 被引量:1
  • 8Wang XS,Chen YY,Shang XF,et al.ldazoxan attenuates spinal cord injury by enhanced astrocytic activation and reduced microglial activation in rat experimental autoimmune encephalomyelitis[J].Brain Res,2009,1253:198-209. 被引量:1
  • 9王新施,郑荣远,陈艳艳.咪唑克生对实验性自身免疫性脑脊髓炎的行为、病理和免疫的影响[J].中国临床神经科学,2007,15(6):570-577. 被引量:11
  • 10Li XF,Kinsey-Jones JS,Knox AM,et al.Neonatal lipopolysac-charide exposure exacerbates stress-induced suppression of iuteinizing hormone pulse frequency in adulthood[J].Endocrinology,2007,148:5984-5990. 被引量:1

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