摘要
目的探讨哮喘小鼠发病过程中基质细胞衍生因子-1(SDF-1)及其受体CXCR4在肺内表达的变化及布地奈德的干预影响。方法清洁级雄性BALB/c小鼠30只,随机分为对照组、干预组及哮喘组。观察卵蛋白激发及用布地奈德干预后哮喘小鼠气道的病理变化;免疫组织化学染色观察SDF-1表达的变化;RT-PCR法观察CXCR4mRNA表达的变化。结果SDF-1及CXCR4mRNA在对照组中呈低表达,在哮喘组中表达明显增加,使用布地奈德进行干预后SDF-1及CXCR4 mRNA的表达明显降低(0.426±0.052 vs 0.361±0.065;0.829±0.027 vs 0.723±0.094;P<0.05),且二者表达量均与气道壁厚度呈正相关(r=0.744,P<0.01;r=0.553,P<0.01)。结论SDF-1及其受体CXCR4可能参与了哮喘小鼠气道重塑过程,布地奈德干预改善哮喘小鼠的气道重塑可能与降低SDF-1及CXCR的表达有关。
Objective To study the expression of stromal cell derived factor-1（SDF-1） and CXC chemokine receptor 4 （CXCR4） in the airway and the effect of budesonide on their expression in mice with asthma.Methods Thirty BALB/c male mices were randomly divided into three groups： placebo control, untreated asthma, and budesonide-treated asthma. The asthma group were induced by intraperitoneal injection of 10% ovalbumin （OVA ） on days 1, 8 and 15, and then from days 22 to 34, challenged by inhalation of 2% OVA aerosol every other day. The budesonide-treated asthma group received an inhalation of budesonide （1 mg ） before OVA challenge. The pathological changes of the airway were assessed by hematoxylin and eosin staining. The immunohistochemistry was used to estimate the expression of SDF-1 in the lung. RT-PCR was used to evaluate the expression of CXCR4 in the lung.Results Compared with the control group, SDF-1 and CXCR4 expression in the lung in the untreated asthma group increased significantly （P0.05）. The budesonide-treated asthma group demonstrated significantly decreased SDF-1 （0.426 ±0.052 vs 0.361±0.065;P0.05） and CXCR4 （0.829 ±0.027 vs 0.723±0.094; P0.05） expression in the lung as compared with the untreated asthma group. Both SDF-1 （r=0.744,P0.01） and CXCR4 （r=0.553, P 0.01） were positively correlated with the thickness of the airway wall.Conclusions SDF-1 and CXCR4 may be associated with airway remodeling in mice with asthma. Budesonide can improve airway remodeling, possibly by decreasing the expression of SDF-1 and CXCR4.
出处
《中国当代儿科杂志》
CAS
CSCD
北大核心
2010年第3期215-218,共4页
Chinese Journal of Contemporary Pediatrics
