摘要
出发菌GR001通过形态观察、生理生化实验、16S rDNA同源性序列分析及部分特异性基因序列分析鉴定为解淀粉芽孢杆菌.以GR001为出发菌,通过化学诱变选育出一株遗传标记为腺嘌呤营养缺陷、6–巯基嘌呤抗性以及蛋氨酸亚砜抗性(Ade-+MSOr+6-MPr)的突变株GR600.以GR600为受体菌,采用原生质体转化法获得转化子GR607(Ade-+MSOr+6-MPr+kmr),鸟苷产量高达20.82,g/L.
According to the characteristics of morphology, physiology and biochemistry experiment and the comparison of 16S rDNA and pur-operon sequence, original strain GR001 was identified as Bacillus amyloliquefaciens. A mutant GR600 （Ade^- ＋ MSO^r＋6-MP^r） was obtained from GR001 by DES treatment. GR600 as a recipient, with the method Of protoplast transformation, a transformant GR607 （Ade ^-＋ MSO^r＋6-Mp^r ＋ km^r） was obtained, guanosine production can reach 20.82 g/L.
出处
《天津科技大学学报》
CAS
2010年第2期1-4,共4页
Journal of Tianjin University of Science & Technology
基金
国家科技重大专项课题(2008ZX09401-05)
关键词
鸟苷
解淀粉芽孢杆菌
诱变
原生质体
guanosine
Bacillus amyloliquefaciens
mutation
protoplast
