摘要
目的观察英夫利西单抗(Infliximab)对前脑缺血再灌注大鼠脑P-糖蛋白(P-gp)蛋白水平的影响。方法8～10月龄30只雄性Wistar大鼠随机分为假手术对照组、单纯脑缺血组和英夫利西单抗组,每组各10只,术后1w取材。采用短暂结扎双侧颈总动脉5min建立CVI模型。免疫组化分析海马区P-gp的表达。结果HE染色显示英夫利西单抗组缺血性改变明显减轻。免疫组化半定量分析表明,与假手术对照组相比,单纯脑缺血组P-gp表达显著升高(P<0.01)。英夫利西单抗组较单纯缺血组显著降低(P<0.01),略高于对照组水平。结论英夫利西单抗抑制脑缺血后P-gp阳性表达,可能有一定保护作用。
Objective To evaluate the effects of the tumor necrosis factor alpha monoantiboy,infliximab,on the p-glycoprotein（P-gp）expression in rat hippocampus after forebrain ischemia. Methods 30 male Wistar rats between eight to ten months were randomly assigned to pseudo-operation control group,forebrain ischemia group,and forebrain ischemia plus infliximab group. The forebrain ischemia model was set up by five-minute occlusion of both common carotid arteries. The subgroups were sacrificed on the seventh day after the operation. P-gp positive neuronal cells were semi-evaluated by immunohistochemistry（IHC）. Results HE stain indicated the infliximab group had less ischemic impairment. Compared to pseudo-operation group,forebrain ischemia group showed upregulated P-gp expression significantly（P0.01）. However,infliximab group had much less P-gp positive than forebrain ischemia group（P0.01）,similar as the pseudo-operation group. Conclusion Infliximab can inhibit the expression of P-gp,which could be neuroprotective during ischemia.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2010年第3期203-204,共2页
Journal of Apoplexy and Nervous Diseases
基金
广东省医学科学技术研究基金(B2007148)
广州市留学归国人员启动基金(2007GZ003)
广州市医药卫生科技基金(2007-YB-006)
