摘要
目的观察FK506对BXSB狼疮性肾炎小鼠治疗作用,初步探讨其作用机制。方法 6只12周龄雄性C57BL/6小鼠为正常对照组,12只12周龄雄性BXSB小鼠随机分为LN治疗组和LN未治疗组。治疗组予FK506 2 mg/kg隔日腹腔注射,未治疗组、正常对照组予NS 0.2 ml隔日腹腔注射,治疗至20周龄。PASM染色法观察肾组织病理变化,直接免疫荧光法观察肾组织IgG沉积,ELISA法检测血清抗-dsDNA抗体和IL-18水平,RT-PCR法检测肾组织IL-18 mRNA表达水平,免疫组化法检测肾组织IL-18表达水平。结果治疗组小鼠肾脏病理学改变明显好转,肾组织免疫球蛋白沉积减少,血清抗-dsDNA抗体和IL-18水平、肾组织IL-18mRNA和蛋白表达水平均低于未治疗组。结论 FK506对BXSB小鼠肾损害具有良好的治疗作用,抑制IL-18 mRNA的表达可能是FK506治疗LN的作用机制之一。
To study the therapeutical effects of FK506 on BXSB mice with lupus nephritis （LN） and primarily evaluate the mechanism. With six 12-weeek-old male C57BL/6 mice acted as normal control group, twelve 12-weeek-old male LN BXSB mice were randomly divided into treated and untreated LN groups. The treated group was given 2 mg/kg FK506 by intraperitoneal injection every other day for eight weeks. While the untreated group and control group were given 0.2 ml saline by the same route and interval. All mice were sacrificed after 8 weeks. The pathological feature was observed by PASM staining; IgG deposition was detected by direct immnnofluoreseence; sera levels of anti-ds-DNA antibody and IL-18 were measured by ELISA; the renal expression of IL-18mRNA was detected by RT-PCR; IL-18 protein expression in kidney was determined by immunohistoehemistry. Compared with the untreated group, the pathological feature were improved, the deposition of IgG was reduced, the serum expressions of anti-dsDNA antibody and IL-18 were decreased, and the renal expression of IL -18 protein and mRNA were all reduced significantly in treated group. In the light of these results, we deduced that inhibiting IL-18 mRNA expression maybe one of the mechanisms to explain the therapeutical effects of FK506 on LN BXSB mice.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2010年第2期112-115,共4页
Immunological Journal
