摘要
以红酵母菌株NZ-01为试验菌株,研究其发酵工艺与中试生产。采用摇瓶发酵优化的方式,研究培养基组分与发酵工艺条件对该菌发酵的影响,并进行中试放大生产。结果显示,该菌最适生长培养基组分为葡萄糖10g/L,蔗糖10g/L,酵母膏10g/L,牛肉膏2.5g/L;色素合成最适培养基组分为葡萄糖15g/L,蔗糖10g/L,酵母膏2.5g/L,牛肉膏5g/L。最适生长起始pH值为6.0,最适接种量为8%,生长周期为44h;最适色素合成起始pH值为7.0,最适色素合成接种量为8%,色素合成周期为48h。发酵优化后的色素产量3.88μg/mL较优化前1.71μg/mL提高了127%。中试产量达3.05μg/mL。红酵母菌NZ-01优化后的发酵条件可以应用于中试生产虾青素,有规模化生产应用潜力。
The fermentation optimization and pilot production of red yeast NZ-01 was studied.The effects of the culture medium components and the fermentation conditions on the yeast growth and pigment yield were investigated by shake flask.The pilot fermentation experiment was carried out.The results indicated that the optimum culture medium for growth consisted of glucose 10 g/L,sucrose 10 g/L,yeast extract10 g/L,Beef extract 2.5 g/L and the optimum culture medium for pigment producing consisted of Glucose 15 g/L,sucrose 10 g/L,Yeast extract 2.5 g/L,Beef extract 5.0 g/L.The optimum fermentation conditions for red yeast NZ-01 growth were incubation of 44 h,initial pH value 6.0 and inoculum＇s level 8 %（v/v）.The optimum fermentation conditions for red yeast NZ-01 pigment producing were incubation of 48 h,initial pH value 7.0 and inoculum＇s level 8 %（v/v）.There was an overall increase of 127% pigment producing yield （3.88 μg/mL）compared with that of the original conditions （1.71 μg/mL）.The pigment yield reached 3.05 μg/mL after pilot fermentation.Therefore,we can conclude that the parameters screened by shake flask could be applied to NZ-01 pigment in pilot scale directly.Red yeast NZ-01 pigment had the potential to produce at large scale.
出处
《生物技术通报》
CAS
CSCD
北大核心
2010年第4期198-202,共5页
Biotechnology Bulletin
基金
国家民委科研项目(08XBE09)
宁夏自然科学基金项目(NZ0841)
关键词
红酵母
虾青素
发酵优化
Red yeast Astaxanthin Fermentation optimization
