摘要
目的探讨14-3-3σ基因与乳腺癌发生的相关性。方法采用甲基化特异性聚合酶链反应法对散发性乳腺癌患者癌组织标本进行14-3-3σ甲基化检测,SYBR greenⅠ实时定量PCR法检测14-3-3σmRNA转录水平。结果采用相对量分析法,△△CT为0.77,根据样本平均相对含量(%)=2-△△CT,得出14-3-3σ基因在癌症组平均转录表达量是非癌组的58%。14-3-3σ基因启动子在42例散发性乳腺癌标本中甲基化率为66.7%,在24例乳腺非癌组织中甲基化率为25%,二者差异有显著性(2=10.616,P<0.05)。结论14-3-3σ基因在乳腺癌组织中有较高的甲基化率,且mRNA表达水平降低,可能甲基化的异常影响了14-3-3σ基因的表达,从而促进乳腺癌的发生。
Objective To study the methylation and mRNA expression of 14-3-3σ gene in sporadic breast cancer and to investigate the correlation between 14-3-3σ gene and breast cancer.Methods The methylation of 14-3-3σgene in breast cancer tissues was examined by methylation-specific polymerase chain reaction（MSP）.The mRNA expression was assessed using SYBR green Ⅰreal time PCR.Results The average expression level of 14-3-3σ gene in cancer tissue was 58% in relative amount analysis.Methylation rates of 14-3-3σgene in cancer group and control group were 66.7% and 25%（X2=10.616,P0.05）,respectively.Conclusion Higher methylation rate and lower expression level of 14-3-3σgene occur in breast cancer.Abnomal methylation of 14-3-3σgene may affect its expression and promote breast carcinogenesis
出处
《广东医学》
CAS
CSCD
北大核心
2010年第6期717-719,共3页
Guangdong Medical Journal
基金
广东省深圳市科技计划项目(编号:200803003)
