摘要
目的探讨重组人γ干扰素（ｒｈＩＦＮγ）对人胚胎胰岛分泌白细胞介素６（ＩＬ６）以及对胰岛分泌功能的影响，为ＩＦＮγ和ＩＬ６在１型糖尿病中的可能作用提供依据。方法采用胶原酶Ｖ型消化方法分离人胚胎胰岛，并对其进行原代培养，加入不同浓度的ｒｈＩＦＮγ培养４８小时收获上清，进行ＩＬ６、胰岛素、胰升血糖素活性检测和ＩＬ６单抗中和试验，对ｒｈＩＦＮγ刺激和对照组胰岛ＲＮＡ进行ＩＬ６ＲＮＡ斑点杂交。结果（１）ｒｈＩＦＮγ刺激后胰岛培养上清ＩＬ６含量显著升高，当ｒｈＩＦＮγ浓度在１００ｕ／ｍｌ时ＩＬ６含量最高；（２）ＩＬ６单抗对ｒｈＩＦＮγ刺激的胰岛培养上清中ＩＬ６有很强的中和效应；（３）斑点杂交结果显示ｒｈＩＦＮγ刺激的胰岛ＲＮＡ中ＩＬ６ｍＲＮＡ含量高于对照组；（４）人胚胎胰岛受ｒｈＩＦＮγ刺激后培养上清胰岛素降低不明显；（５）人胚胎胰岛受ｒｈＩＦＮγ刺激后培养上清胰升血糖素分泌能力明显升高，分泌趋势与ＩＬ６相似。结论ｒｈＩＦＮγ可以促进胰岛分泌ＩＬ６，而ＩＬ６的过度分泌可以加重１型糖尿病的进程。
Objective To study effects of rhIFNγ on fetal islet function and IL 6 prodction in vitro. Methods The islets from fetal pancreas were separated by collagenase type V (0.5mg/ml) and cultured in vitro. The islets were exposed for 48 hours to culture medium alone or with different concentrations of rhIFNγ. The supernatants of culture of human fetal islets were assayed for IL 6, insulin and glucagon. Results The results showed: (1)IL 6 activity was increased by 3.24 folds (87 282mU/islet) when islets were exposed to rhIFNγ (100u/ml); (2)IL 6 monoclonal antibody significantly reduced the IL 6 activity in islet supernatants from control group or from islets exposed to rhIFNγ; (3)IL 6 mRNA in human fetal islets exposed to rhIFNγ was higher than that in control in dot hybridization; (4)Soluble insulin released to supernatants was slightly decreased (0.71 0.57 IU/islet) and cellular insulin within islets slightly increased (0.75 0.85 IU/islet); (5)Glucagon secretion was significantly increased by 3.2 folds (1.02 3.3pg/islet). Conclusion IL 6 production by pancreatic islets is up regulated by rhIFNγ, and IL 6 may act as a costimulator for autoreactive B and T lymphocytes in autoimmune diabetes.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
1998年第4期236-239,共4页
Chinese Journal of Endocrinology and Metabolism
