摘要
目的探讨纤粘连蛋白、层粘连蛋白、Ⅰ型胶原３种细胞外基质蛋白促牛晶状体上皮细胞（ＬＥＣ）的粘附作用，从细胞生物学方面阐述后囊膜混浊（ＰＣＯ）发生的病理学机制。方法应用３Ｈ—ＴＤＲ掺入法研究纤粘连蛋白、层粘连蛋白、Ｉ型胶原和鸡卵白蛋白对体外培养的牛ＬＥＣ的粘附作用。结果牛ＬＥＣ能够粘附到包被有不同浓度的纤粘连蛋白、层粘连蛋白、Ⅰ型胶原培养板表面上，呈现出浓度和时间效应特点；但牛ＬＥＣ不能够粘附到包被有不同浓度的鸡卵白蛋白培养板表面上。结论纤粘连蛋白、层粘连蛋白、Ⅰ型胶原能够介导ＬＥＣ粘附到后囊膜上，在ＰＣＯ发生过程中它们可起到重要的促进作用。
ObjectiveTo inquire into the role of fibronectin,laminin and type I collagen in promotion of bovine lens epithelial cells(LEC)adhesion and to explain the pathogenesis of posterior capsular opacification(PCO).Methods 3H TDR incorporating assays were applyed to study adhesion of bovine LEC cultued in response of fibronectin,laminin,type I collagen and ovalbumin in vitro.ResultsBovine LEC adhered to surfaces coated with varying concentrations of fibronectin,laminin and type I collagen,and the adhesion of the cells was dependent on the concentration of coated substates and time.Bovine LEC did not adhere to ovalbumin coated surfaces at any concentration tested.ConclusionThe results of these studies indicate that adhesion of LEC occurs in response to fibronectin,laminin and type I collagen,which may play a critical promotive role in the occurrence of PCO.
出处
《眼科研究》
CSCD
1998年第4期259-261,共3页
Chinese Ophthalmic Research
关键词
晶状体上皮细胞
细胞外基质蛋白
LEC
PCO
ens epithelial cells posterior capsular opacification fibronectin laminin type I collagen
