摘要
目的探讨装配线工人职业紧张与唾液考的松、分泌型免疫球蛋白A(sIgA)及溶菌酶水平的关系。方法采用自然分泌方式收集每名研究对象1个工作日的5份唾液样品,收集时间为班前基础样品(7∶30),上午班中样品(9∶30),中午工间休息样品(12∶30),下午班中样品(15∶00)和班后恢复期样品(17∶00);同时每名研究对象完成1份职业紧张调查问卷,问卷主要调查人口统计学特征资料、职业紧张因素、身心健康状况和人格特征。唾液考的松、sIgA和溶菌酶浓度分别采用放射免疫、免疫放射和比浊法分析测定。使用相关分析、多元逐步回归分析和重复测量方差分析对资料进行统计学处理。结果基础和/或平均唾液考的松、sIgA和溶菌酶浓度与某些职业紧张因素、紧张结局及人格特征评分呈显著性负相关(P<0.05或P<0.01);一些心理变量对基础和/或平均唾液考的松、sIgA和溶菌酶浓度具有显著性预测作用(R2>0.05,P<0.05或P<0.01);唾液sIgA和溶菌酶浓度与唾液考的松浓度无相关关系(P>0.05)。工作需求引起了唾液考的松、sIgA和溶菌酶浓度的显著上升。结论唾液sIgA和溶菌酶浓度对心理紧张是敏感的,二者可以作为职业紧张现场调查评价指标。
Objective To investigate the association between occupational stress and concentrations of salivary cortisol,sIgA and lysozyme.Methods5 saliva samples over a work day were collected from 50 male refrigerator assembly line workers,who completed a self-reported questionnaire to assess the occupational stress.Concentrations of salivary cortisol,sIgA,and lysozyme were measured by radioimmunoassay,immunoradiometricassay and lyso-plate method respectively.ResultsThe results revealed that baseline and/or mean concentrations of salivary cortisol,sIgA and lysozyme showed significantly negative correlation with some occupational stressors,self-reported health and personality scores.Some psychological variables showed significant predictive effects on salivary indicators.The salivary sIgA and lysozyme were not correlated with the salivary cortisol.The working demand induced a marked increase in the concentrations of salivary IgA and lysozyme,as well as salivary cortisol.ConclusionsSalivary IgA and lysozyme concentrations are sensitive to psychological stress and could be utilized as potential biomarkers in field investigation of the effects of stress on immunity.
出处
《工业卫生与职业病》
CAS
CSCD
北大核心
2010年第2期99-104,共6页
Industrial Health and Occupational Diseases
基金
河南省医学科技创新人才工程计划项目(2001-23
2004-62)
